Penson S. P., Schuurink R. C., Fath A., Gubler F., Jacobsen J. V., Jones R. L.
Department of Plant Biology, University of California-Berkeley, Berkeley, California 94720-3102.
Plant Cell. 1996 Dec;8(12):2325-2333. doi: 10.1105/tpc.8.12.2325.
The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain. Levels of cGMP in freshly isolated barley aleurone layers ranged from 0.065 to 0.08 pmol/g fresh weight of tissue, and cGMP levels increased transiently after incubation in gibberellic acid (GA). Abscisic acid (ABA) did not increase cGMP levels in aleurone layers. LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP and inhibited GA-induced [alpha]-amylase synthesis and secretion. The inhibitory effects of LY could be overcome by membrane-permeant analogs of cGMP. LY also prevented GA-induced accumulation of [alpha]-amylase and GAMYB mRNAs. cGMP alone was not sufficient to induce the accumulation of [alpha]-amylase or GAMYB mRNA. LY had a less dramatic effect on the accumulation of mRNAs encoding the ABA-responsive gene Rab21. We conclude that cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell.
对大麦（喜马拉雅品种）种子糊粉层和原生质体中cGMP的产生及作用进行了研究。新鲜分离的大麦糊粉层中cGMP水平在0.065至0.08 pmol/g组织鲜重之间，在赤霉素（GA）处理后cGMP水平短暂升高。脱落酸（ABA）未使糊粉层中的cGMP水平升高。鸟苷酸环化酶抑制剂LY 83583（LY）可阻止GA诱导的cGMP增加，并抑制GA诱导的α淀粉酶合成与分泌。cGMP的膜通透性类似物可克服LY的抑制作用。LY还可阻止GA诱导的α淀粉酶和GAMYB mRNA积累。单独的cGMP不足以诱导α淀粉酶或GAMYB mRNA积累。LY对ABA响应基因Rab21编码的mRNA积累影响较小。我们得出结论，cGMP在大麦糊粉层细胞的GA信号传导中起重要作用，但在ABA信号传导中不起作用。
