Świeżawska-Boniecka Brygida, Szmidt-Jaworska Adriana
Department of Plant Physiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Toruń, Poland.
Front Plant Sci. 2025 Jun 25;16:1618243. doi: 10.3389/fpls.2025.1618243. eCollection 2025.
This review examines the historical trajectory of cyclic nucleotides (cNMPs) research in plants, a field that has encountered prolonged challenges and skepticism, contrasting sharply with its rapid validation in animal systems. For decades, efforts to detect cyclic nucleotides, specifically cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), in plant tissues and to elucidate their functional roles were inconclusive. These challenges primarily stemmed from the extremely low endogenous concentrations of cNMPs in plant cells, the rapid turnover of these molecules, and the limited sensitivity and specificity of early analytical techniques. However, since the 1970s, significant advances in analytical methodologies and bioinformatics have enabled precise quantification of cNMP levels and the bioinformatics identification of enzymes central to plant cNMP signaling pathways. In this review, we trace the key milestones and transformative discoveries that have shaped the evolving landscape of cyclic nucleotide research in plants, highlighting how each step forward has deepened our understanding of cAMP and cGMP as integral regulators of plant physiology.
本综述考察了植物中环状核苷酸（cNMPs）研究的历史轨迹，该领域长期面临挑战和质疑，这与它在动物系统中迅速得到验证形成鲜明对比。几十年来，在植物组织中检测环状核苷酸，特别是环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）并阐明其功能作用的努力一直没有定论。这些挑战主要源于植物细胞中cNMPs的内源性浓度极低、这些分子的快速周转以及早期分析技术的灵敏度和特异性有限。然而，自20世纪70年代以来，分析方法和生物信息学的重大进展使得能够精确量化cNMP水平，并通过生物信息学鉴定植物cNMP信号通路的关键酶。在本综述中，我们追溯了塑造植物环状核苷酸研究不断演变格局的关键里程碑和变革性发现，强调每一步进展如何加深了我们对cAMP和cGMP作为植物生理学重要调节因子的理解。
