Conconi Maria Teresa, Tommasini Mara, Baiguera Silvia, De Coppi Paolo, Parnigotto Pier Paolo, Nussdorfer Gastone G
Department of Pharmaceutical Sciences, University of Padua, I-35121 Padua, Italy.
Int J Mol Med. 2002 Oct;10(4):451-6.
Prostaglandins E (PGEs) are abundantly produced in the skeletal tissue, the turnover of which they can modulate acting on both bone deposition and resorption. We compared the effects of PGE1 and PGE2 on the growth and differentiation of rat bone-marrow osteoblast-like cells cultured in vitro. Both PGEs stimulated cultured cell growth, PGE2 being more effective than PGE1. PGE1 inhibited and PGE2 enhanced alkaline phosphatase activity. Both PGEs markedly raised osteocalcin synthesis, without apparently affecting collagenase-digestible protein production. Scanning electron microscopy showed that untreated cultured osteoblast-like cells were arranged in clusters and displayed a polygonal shape. PGE1 did not alter cell morphology, while PGE2 provoked elongation of cultured cells and sprouting of slend cytoplasmic processes. Morphometric analysis indicated that PGE1 decreased and PGE2 increased cultured-cell dimensions. Collectively, these findings allow us to conclude that PGE1 and PGE2, although being both able to enhance proliferation of osteoblast-like cells cultured in vitro, exert divergent effects on their differentiation. PGE1 seems to slow-down osteoblast maturation, while PGE2 appears to stimulate osteoblast differentiation to mature osteocytes.
前列腺素E（PGEs）在骨骼组织中大量产生，它们可以通过作用于骨沉积和骨吸收来调节其更新。我们比较了PGE1和PGE2对体外培养的大鼠骨髓成骨细胞样细胞生长和分化的影响。两种PGEs均刺激培养细胞的生长，其中PGE2比PGE1更有效。PGE1抑制而PGE2增强碱性磷酸酶活性。两种PGEs均显著提高骨钙素的合成，而对胶原酶可消化蛋白的产生无明显影响。扫描电子显微镜显示，未经处理的培养成骨细胞样细胞呈簇状排列，呈多边形。PGE1未改变细胞形态，而PGE2促使培养细胞伸长并长出细长的细胞质突起。形态计量分析表明，PGE1使培养细胞尺寸减小，而PGE2使其增大。总的来说，这些发现使我们得出结论，PGE1和PGE2虽然都能增强体外培养的成骨细胞样细胞的增殖，但对其分化产生不同的影响。PGE1似乎减缓了成骨细胞的成熟，而PGE2似乎刺激成骨细胞分化为成熟的骨细胞。
