Schönmann M J, BonDurant R H, Gardner I A, Van Hoosear K, Baltzer W, Kachulis C
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis 95616.
Vet Rec. 1994 Jun 11;134(24):620-2. doi: 10.1136/vr.134.24.620.
Sampling methods for preputial secretions and culture methods for Tritrichomonas foetus were compared in a two-by-two factorial experiment. Fourteen bulls were confirmed as infected by repeated weekly sampling and culture on Diamond's medium over the course of 10 months. In a six-week test of alternative methods of diagnosis, the bulls were randomly assigned to have their preputial contents sampled weekly either by washing or by scraping of the preputial membrane. The samples were cultured on Claussen's medium and in InPouch packets at 37 degrees C in air for seven days. The results were compared by McNemar's chi 2 test for symmetry. Washing yielded 69 positive cultures from 84 samples whereas scraping yielded 65 from 83. These differences were not statistically significant. InPouch culture detected 73 of 83 samples from positive bulls, whereas Claussen's medium detected 61 of 83. The difference in sensitivity between the two culture methods was highly significant (P = 0.0027), with the InPouch method 'missing' two positive samples that were detected by Claussen's medium and Claussen's medium missing 14 positive samples that were detected by InPouch culture.
在一项二乘二析因实验中，对包皮分泌物的采样方法和胎儿三毛滴虫的培养方法进行了比较。通过在10个月的时间里每周重复采样并在戴蒙德培养基上培养，确认了14头公牛受到感染。在一项为期六周的诊断替代方法测试中，公牛被随机分配，每周通过冲洗或刮擦包皮膜来采集包皮内容物样本。样本在克劳森培养基上以及在InPouch培养袋中于37摄氏度的空气中培养7天。通过麦克尼马尔卡方检验进行对称性比较结果。冲洗84个样本得到69个阳性培养物，而刮擦83个样本得到65个阳性培养物。这些差异无统计学意义。InPouch培养法从阳性公牛的83个样本中检测出73个，而克劳森培养基从83个样本中检测出61个。两种培养方法在敏感性上的差异非常显著（P = 0.0027），InPouch方法“漏检”了克劳森培养基检测出的2个阳性样本，克劳森培养基则漏检了InPouch培养法检测出的14个阳性样本。
