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使用两种采样方法和一种运输培养基诊断公牛胎儿三毛滴虫感染

Diagnosis of Tritrichomonas foetus infection in bulls using two sampling methods and a transport medium.

作者信息

Tedesco L F, Errico F, Del Baglivi L P

出版信息

Aust Vet J. 1979 Jul;55(7):322-4. doi: 10.1111/j.1751-0813.1979.tb00418.x.

DOI:10.1111/j.1751-0813.1979.tb00418.x
PMID:391209
Abstract

Preputial exudates were collected from 3 bulls infected with Tritrichomonas foetus by scraping the mucosa with a specially designed instrument and by aspiration. For diagnostic purposes the scraping method was superior direct microscopic examination but both methods were equally good when the samples were cultured within 2 hours of collection. The organism remained viable in a transport medium for 24, 48 and 72 hours showing a lineal decrease in viability with time which was more than 3 times greater in samples aspirated than in samples scraped.

摘要

通过用特制器械刮擦黏膜以及抽吸的方式,从3头感染胎儿三毛滴虫的公牛采集包皮渗出物。用于诊断目的时,刮擦法优于直接显微镜检查,但当样本在采集后2小时内进行培养时,两种方法同样有效。该生物体在运输培养基中可存活24、48和72小时,其活力随时间呈线性下降,抽吸样本中的活力下降幅度比刮擦样本大3倍以上。

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