Hajhashemi Valiollah, Ghannadi Alireza, Pezeshkian Sayed Karim
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
J Ethnopharmacol. 2002 Oct;82(2-3):83-7. doi: 10.1016/s0378-8741(02)00137-x.
Satureja hortensis L. (Lamiaceae) is a medicinal plant used in Iranian folk medicine as muscle and bone pain reliever. In the present study, hydroalcoholic extract, polyphenolic fraction and essential oil of the aerial parts of the herb were prepared and evaluated for the analgesic activity using light tail flick, formalin and acetic acid-induced writhing in mice. Also, the anti-inflammatory effects of the above-mentioned preparations were assessed using carrageenan-induced paw edema in rats. Results showed that in the light tail flick test neither the essential oil nor the extracts could exert any significant effect. The hydroalcoholic extract (2000 mg/kg, p.o.) and the essential oil (200 mg/kg, p.o.) inhibited the mice writhing responses caused by acetic acid. In formalin test, hydroalcoholic extract (500-2000 mg/kg, p.o.), polyphenolic fraction (250-1000 mg/kg, p.o.) and the essential oil (50-200 mg/kg, p.o.) showed analgesic activity and pretreatment with naloxone (1 mg/kg, i.p.) or caffeine (20 mg/kg, i.p.) failed to reverse this antinociceptive activity. Polyphenolic fraction (1000 mg/kg, p.o.) and the essential oil (200 mg/kg) reduced edema caused by carrageenan. These results suggest that S. hortensis L. has antinociceptive and anti-inflammatory effects and probably mechanism(s) other than involvement of opioid and adenosine receptors mediate(s) the antinociception.
香薄荷（唇形科）是一种药用植物，在伊朗民间医学中用作肌肉和骨骼疼痛缓解剂。在本研究中，制备了该草药地上部分的水醇提取物、多酚组分和精油，并使用小鼠光尾甩、福尔马林和乙酸诱导扭体法评估其镇痛活性。此外，使用角叉菜胶诱导大鼠足跖肿胀评估上述制剂的抗炎作用。结果表明，在光尾甩试验中，精油和提取物均未产生任何显著作用。水醇提取物（2000mg/kg，口服）和精油（200mg/kg，口服）抑制了乙酸引起的小鼠扭体反应。在福尔马林试验中，水醇提取物（500 - 2000mg/kg，口服）、多酚组分（250 - 1000mg/kg，口服）和精油（50 - 200mg/kg，口服）显示出镇痛活性，用纳洛酮（1mg/kg，腹腔注射）或咖啡因（20mg/kg，腹腔注射）预处理未能逆转这种抗伤害感受活性。多酚组分（1000mg/kg，口服）和精油（200mg/kg）减轻了角叉菜胶引起的水肿。这些结果表明，香薄荷具有抗伤害感受和抗炎作用，并且可能是除阿片类和腺苷受体参与之外的其他机制介导了抗伤害感受。
