Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Med Princ Pract. 2012;21(2):178-82. doi: 10.1159/000333555. Epub 2011 Nov 16.
The aim of this study was to evaluate the anti-inflammatory and analgesic effects of Satureja hortensis seed extracts and to analyze their essential oil constituents.
Hydroalcoholic and polyphenolic extracts and essential oil of S. hortensis seeds were prepared using standard methods. Analgesic activity was assessed in male mice (25-35 g) using standard methods (acetic acid and formalin tests). For the evaluation of anti-inflammatory activity, the carrageenan-induced rat paw edema test was used. The mice were pretreated with 50, 100 or 200 mg/kg, i.p., hydroalcoholic or polyphenolic extracts or 100 or 200 μl/kg, p.o.
Hydrodistillation of the seeds of S. hortensis afforded a pale yellowish oil in a yield of 0.05% (v/w). Pretreatment of mice with hydroalcoholic or polyphenolic extracts or essential oil significantly (p < 0.001) reduced acetic acid-induced abdominal twitches. Hydroalcoholic extracts also significantly reduced pain responses in early and late phases of the formalin test whereas the polyphenolic extract and essential oil were only effective in the late phase of the formalin test. All three fractions were found to reduce paw edema in the carrageenan test.
These results clearly demonstrate the analgesic and anti-inflammatory activity of S. hortensis seeds and since the hydroalcoholic extract relieved pain in the first phase of the formalin test, it seems that at least part of its analgesic activity may be mediated centrally. The results of this study substantiated the traditional use of S. hortensis plant seeds in painful and inflammatory ailments.
本研究旨在评估香荆芥籽提取物的抗炎和镇痛作用，并分析其精油成分。
采用标准方法制备香荆芥籽的水醇提物和多酚提取物及精油。采用标准方法（醋酸和福马林试验）评估雄性小鼠（25-35g）的镇痛活性。为评估抗炎活性，采用角叉菜胶诱导的大鼠足肿胀试验。小鼠预先用 50、100 或 200mg/kg，ip 给予水醇提物或多酚提取物或 100 或 200μl/kg，po 预处理。
香荆芥籽经水蒸馏得到淡黄色油，产率为 0.05%（v/w）。水醇提物或多酚提取物或精油预处理可显著（p<0.001）减少醋酸诱导的腹部抽搐。水醇提物还显著降低福马林试验早期和晚期的疼痛反应，而多酚提取物和精油仅在福马林试验晚期有效。三种馏分均能降低角叉菜胶试验中的足肿胀。
这些结果清楚地表明香荆芥籽具有镇痛和抗炎活性，由于水醇提物缓解了福马林试验第一阶段的疼痛，因此其至少部分镇痛活性可能是通过中枢介导的。本研究结果证实了香荆芥植物种子在疼痛和炎症性疾病中的传统用途。
