Bosković S, Lucić N, Aganović N, Grbić E
Bilt Hematol Transfuz. 1975;3(3):69-77.
In premises for blood conservation, production of dry human plasma and infusion solutions "notwithstanding the permanent measures for desinfection, new bacterial contamination occurs from time to time and whose source are the casings and material originating from non-sterile environment. Bacteriological control, which has primarily a preventive character, enables a due forecast for measures to be undertaken by the appropriate desinfection of the working surfaces and air, satisfactory conditions of aseptic work can be maintained. General hygiene should be paid attention to as well as mechanical cleansing of premises, avoidance of groups for lunch-time etc., since the treatment by desinfectors would not be sufficient for maintenance of aseptic working conditions. In order to prevent the transmission of bacterial contamination, premises for blood conservation should be strictly separated from other operations and also prevent the unnecessary movements of personnel through corridors. The results of the bacteriological control of the personnel show that greater attention should be paid to their health care since the workers there work in closed aseptic systems and thus avoid them as a bacteria transmittors in respect to danger of blood and dry human plasma contamination. It is also necessary to efficiently educate the personnel for work in aseptic conditions and also increase their elementary knowledge from bacteriology and hygiene. The bacterial skin-flora on the spot of donor's venepuncture also presents a certain danger for blood contamination. Therefore, it is necessary to establish the most optimal manner of skin desinfecate together with the most appropriate means having a fast bactericidal and fungicidal action. It would also be useful, on the basis of further test, to suggest certain standard for an allowed number of conditionally pathogenic and saprophytic microorganisms which would be used by the instutions performing the blood transfusion and production of the intravenous solutions during their bacteriological controls.
在血液保存、冻干人血浆和输液溶液生产场所，“尽管采取了永久性消毒措施，但仍不时出现新的细菌污染，其来源是来自非无菌环境的包装和材料。主要具有预防性质的细菌学控制，能够对通过对工作表面和空气进行适当消毒来采取的措施进行合理预测，从而可以维持令人满意的无菌工作条件。应注意一般卫生以及场所的机械清洁，避免集体午餐等情况，因为仅靠消毒剂处理不足以维持无菌工作条件。为防止细菌污染的传播，血液保存场所应与其他操作严格分开，还应防止人员不必要地在走廊走动。对人员的细菌学控制结果表明，应更加关注他们的医疗保健，因为那里的工作人员在封闭的无菌系统中工作，从而避免他们成为血液和冻干人血浆污染危险的细菌传播者。对人员进行无菌条件下工作的有效培训以及增加他们在细菌学和卫生方面的基础知识也很有必要。献血者静脉穿刺部位的皮肤菌群也对血液污染构成一定危险。因此，有必要确定皮肤消毒的最佳方式以及具有快速杀菌和杀真菌作用的最合适手段。根据进一步测试，提出某些关于允许的条件致病和腐生微生物数量的标准也将是有用的，这将供进行输血和静脉输液生产的机构在其细菌学控制中使用。
