Mach P S, Rebut-Bonneton C, Le Gô A, Piatier D, Delbarre F
Biomedicine. 1975 Nov;22(6):532-8.
In this report is was demonstrated that in rheumatoid factors (RF) positive sera, 19 S IgM rheumatoid factor can form soluble complexes with different proteins (IgG, albumin) of sera. In these complexes the antiglobulin activity of IgM is not inhibited. When immunofluorescence and immunoadsorption procedures are used for the detection of antiglobulin activities of rheumatoid sera, the proteins which are bound to IgM rheumatoid factor, even if they are devoid of any antiglobulin character, may be revealed simultaneously with IgM. Moreover in some cases the detection of IgM may be hindered, while the linked proteins remain detectable. In these conditions, these complexes in RF positive sera may give false negative results for IgM rheumatoid factor, and may give rise to artefactual appearance of IgG and other proteins (albumin antiglobulin-like activities. This paper points out that before investigating IgG and IgA antiglobulin activities, IgM rheumatoid factor should be previously eliminated, for example by immunoadsorption.
本报告表明，在类风湿因子（RF）阳性血清中，19S IgM类风湿因子可与血清中的不同蛋白质（IgG、白蛋白）形成可溶性复合物。在这些复合物中，IgM的抗球蛋白活性未受抑制。当采用免疫荧光和免疫吸附程序检测类风湿血清的抗球蛋白活性时，与IgM类风湿因子结合的蛋白质，即使它们没有任何抗球蛋白特性，也可能与IgM同时显示出来。此外，在某些情况下，IgM的检测可能会受到阻碍，而与之相连的蛋白质仍可检测到。在这些情况下，RF阳性血清中的这些复合物可能会使IgM类风湿因子出现假阴性结果，并可能导致IgG和其他蛋白质（白蛋白抗球蛋白样活性）出现人为假象。本文指出，在研究IgG和IgA抗球蛋白活性之前，应先通过免疫吸附等方法消除IgM类风湿因子。
