Vose B M, Moore M, Schofield P F, Dymock I W
Clin Exp Immunol. 1975 Dec;22(3):393-8.
The survival of cells from five different cultures of allogeneic malignant colonic carcinoma, two from normal adult colonic epithelium and eight from foetal colonic epithelium in the presence of leucocytes from patients with neoplastic and inflammatory disorders of the colon has been compared. Cytotoxicity assessed by the reduction of the number of adherent target cells in microplate wells compared with those surviving in wells treated with tissue culture medium alone was observed with leucocytes from donors in all categories examined including those from individuals without any known abnormality. Patients with ulcerative colitis were the only group to reveal consistent reactivity against cultures derived from all three sources, an observation which may reflect sensitization to organ-related antigens in this disease. In contrast, leucocytes from patients with bowel neoplasia showed reactivity for cells derived from colon carcinoma tissue, which was comparable to that of healthy donors. Evidence for tumour-specific cytotoxicity was therefore lacking in this study. It is suggested that the detection of tumour-associated antigens on cultured cells may be limited by a number of factors of which the wide variation in reactivity among controls and unspecified nature of the target cells are likely to be of greatest importance.
对来自五种不同同种异体恶性结肠癌培养物、两种正常成人结肠上皮培养物以及八种胎儿结肠上皮培养物的细胞，在患有结肠肿瘤性和炎性疾病患者的白细胞存在的情况下的存活率进行了比较。通过与仅用组织培养基处理的孔中存活的细胞相比，微板孔中贴壁靶细胞数量的减少来评估细胞毒性，在所检查的所有类别供体的白细胞中均观察到细胞毒性，包括来自无任何已知异常个体的白细胞。溃疡性结肠炎患者是唯一一组对源自所有三种来源的培养物表现出一致反应性的群体，这一观察结果可能反映了该疾病中对器官相关抗原的致敏作用。相比之下，肠道肿瘤患者的白细胞对源自结肠癌细胞组织的细胞表现出反应性，这与健康供体的反应性相当。因此，本研究缺乏肿瘤特异性细胞毒性的证据。有人提出，培养细胞上肿瘤相关抗原的检测可能受到多种因素的限制，其中对照之间反应性的广泛差异以及靶细胞性质未明确可能是最重要的因素。
