人及蟾蜍胃黏膜中胃蛋白酶、胃促胰酶及其酶原的部分特性研究

Partial characterization of pepsins and gastricsins and their zymogens from human and toad gastric mucosae.

Ward P H, Neumann V K, Chiang L

Department of Physiology, University of Concepción, Chile.

Comp Biochem Physiol B. 1978;61(4):491-8. doi: 10.1016/0305-0491(78)90040-8.

Abstract

Three zymogens have been isolated from human gastric mucosae and two from the stomachs of the toad Caudiverbera caudiverbera. 2. Human zymogens I and III were immunologically related and cross-reacted with antisera prepared against porcine pepsinogen. The third, (II), showed no cross-reactivity. 3. Human zymogens I and III and toad zymogen ZII gave rise to two human pepsins and to a pepsin-like enzyme, respectively. 4. Human zymogen II (gastricsinogen) and toad zymogen ZI gave rise to human gastricsin and to a gastricsin-like enzyme respectively. 5. The toad enzymes showed much greater stability at neutral and alkaline pH values than the human enzymes.

摘要

已从人胃黏膜中分离出三种酶原，从蟾蜍尾蟾的胃中分离出两种酶原。2. 人酶原I和III在免疫上相关，并且与针对猪胃蛋白酶原制备的抗血清发生交叉反应。第三种（II）没有交叉反应。3. 人酶原I和III以及蟾蜍酶原ZII分别产生两种人胃蛋白酶和一种类胃蛋白酶。4. 人酶原II（胃泌酸蛋白酶原）和蟾蜍酶原ZI分别产生人胃泌酸蛋白酶和一种类胃泌酸蛋白酶。5. 蟾蜍的酶在中性和碱性pH值下比人的酶表现出更高的稳定性。