γ-氨基丁酸和牛磺酸与猫中枢神经系统各区域突触体组分的结合。

Binding of gamma-aminobutyric acid and taurine to synaptosomal fractions of regions of the feline CNS.

作者信息

Gadea-Ciria M, Carcía-Gracia M, Camacho J G, Somozoa G, Balfagón G, DeFeudis F V

出版信息

J Neurosci Res. 1975;1(5-6):393-7. doi: 10.1002/jnr.490010507.

Abstract

The "binding" of 3H-gamma-aminobutyric acid (3H-GABA) and 14C-taurine (7 X 10(-5) M) to synaptosomal fractions of the feline CNS was studied using differential centrifugation and double-isotope methods. Of the regions examined, the "binding" of GABA (in nmole/mg protein) occurred to the greatest extent, in cerebral cortex, olfactory bulb, and colliculi and, to a lesser extent, in caudate nucleus, corona radiata, and fornix. Distribution ratios revealed that GABA binding was greater in the cerebral cortex than in caudate nucleus, brain stem, spinal cord, and white matter regions. The binding of taurine (in nmole/mg protein) was lowest in cerebral cortex and highest in brain stem and spinal gray regions. Distribution ratios for taurine indicated that its accumulation was similar in all CNS regions studied, except for slightly higher values for cerebellar cortex and fornix. These results indicated that preferential binding of both GABA and taurine exists among regions of the feline CNS.

摘要

采用差速离心和双同位素方法，研究了³H-γ-氨基丁酸（³H-GABA）和¹⁴C-牛磺酸（7×10⁻⁵M）与猫中枢神经系统突触体组分的“结合”情况。在所检查的区域中，GABA（以纳摩尔/毫克蛋白计）的“结合”在大脑皮质、嗅球和丘脑中程度最高，在尾状核、放射冠和穹窿中程度较低。分布比率显示，大脑皮质中GABA的结合高于尾状核、脑干、脊髓和白质区域。牛磺酸（以纳摩尔/毫克蛋白计）的结合在大脑皮质中最低，在脑干和脊髓灰质区域中最高。牛磺酸的分布比率表明，除小脑皮质和穹窿的值略高外，其在所有研究的中枢神经系统区域中的积累相似。这些结果表明，猫中枢神经系统各区域之间存在GABA和牛磺酸的优先结合。