Wang De-Shou, Kobayashi Tohru, Senthilkumaran Balasubramanian, Sakai Fumie, Sudhakumari Cheni Chery, Suzuki Taiga, Yoshikuni Michiyasu, Matsuda Masaru, Morohashi Ken-ichirou, Nagahama Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Biochem Biophys Res Commun. 2002 Sep 27;297(3):632-40. doi: 10.1016/s0006-291x(02)02252-0.
Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of approximately 1.4kb for DAX1 and of approximately 1.2kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10dah and then significantly up-regulated between 10 and 15dah, whereas the expression of SHP is moderate and consistent during the ontogeny.
分别从尼罗罗非鱼中克隆出具有编码296和258个氨基酸残基开放阅读框的鱼类DAX1和SHP cDNA，以及SHP部分基因片段。对DAX1、SHP和同源EST片段进行系统发育分析表明，DAX1和SHP在基因结构上保守，且存在于所有脊椎动物中。Northern印迹分析检测到DAX1约1.4kb的单一条带和SHP约1.2kb的单一条带。通过RT-PCR进行的组织分布分析表明，鱼类DAX1和SHP mRNA在成体组织中广泛表达，分别在性腺和肝脏中表达最为丰富。在孵化后5至90天（dah）的两性性腺中也检测到了DAX1和SHP。然而，DAX1在5和10 dah时表达较弱，然后在10至15 dah之间显著上调，而SHP在个体发育过程中的表达适中且一致。
