Laboratory of Reproductive Biology, National Institute of Basic Biology, Okazaki, 444-8585, Japan.
Fish Physiol Biochem. 2005 Apr;31(2-3):177-81. doi: 10.1007/s10695-006-0021-6.
Gonadal development and steroidogenesis in teleosts is regulated by two gonadotropic hormones; luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Earlier studies in tilapia have shown that FSH-beta and LH-beta appear by 14 days after hatching (dah), results from the current study corroborate with these previous reports in tilapia. Here we demonstrate the appearance of LH in pituitary between 14 and 20 dah. In addition to this the present study primarily focuses on any possible differences in appearance of LH-beta and FSH-beta immunoreactivity between XX and XY population of Nile tilapia. LH immunoreactivity was found to be lower in pituitary of XX fish when compared to XY fish. The development of FSH-beta immunoreactivity in pituitary of the Nile tilapia is also presented. Overall, it remains to be established what significance these findings on the appearance of gonadotropins hold for sex differentiation in tilapia.
鱼类的性腺发育和类固醇生成受两种促性腺激素调节；促黄体生成素（LH）和促卵泡激素（FSH）。先前对罗非鱼的研究表明，FSH-β和 LH-β在孵化后 14 天（dah）出现，本研究的结果与罗非鱼的先前报告相符。在这里，我们证明了 LH 在垂体中出现在 14 到 20 dah 之间。除此之外，本研究主要集中在 XX 和 XY 尼罗罗非鱼群体之间 LH-β和 FSH-β免疫反应出现的任何可能差异上。与 XY 鱼相比，LH 免疫反应在 XX 鱼的垂体中较低。还介绍了尼罗罗非鱼垂体中 FSH-β免疫反应的发育。总的来说，这些关于促性腺激素出现的发现对罗非鱼的性别分化有什么意义，还有待确定。
