用在伴刀豆球蛋白A-琼脂糖上通过亲和层析制备的人抗体包被的乳胶颗粒检测乙肝表面抗原。

Detection of HBs antigen with latex particles coated with human antibody prepared by affinity chromatography on concanavalin A-sepharose.

作者信息

Ishiyama H, Yasuda J, Okuyama K, Masuda K, Ito H

出版信息

Jpn J Med Sci Biol. 1975 Oct-Dec;28(5-6):269-77. doi: 10.7883/yoken1952.28.269.

DOI:10.7883/yoken1952.28.269

Abstract

Human HBs antibody was isolated by affinity chromatography on HBs antigen absorbed to concanavalin A linked to Sepharose 4B. When a human anti-HBs immunoglobulin preparation obtained by Cohn's cold ethanol fractionation method was used as a starting material, the antibody was concentrated about 10 times in terms of the passive hemagglutination titer with a recovery rate higher than 50%. Latex particles coated with human anti-HBs antibody thus prepared were proved to be useful in detecting HBs antigen in human blood samples. In its sensitivity and in rapidity of its performance, the antibody-coated latex agglutination test seems to be superior to conventional immunodiffusion techniques.

摘要

通过在与琼脂糖4B偶联的伴刀豆球蛋白A吸附的乙肝表面抗原上进行亲和层析，分离出了人乙肝表面抗体。当使用通过科恩冷乙醇分级分离法获得的人抗乙肝表面免疫球蛋白制剂作为起始原料时，该抗体在被动血凝滴度方面浓缩了约10倍，回收率高于50%。如此制备的包被有人抗乙肝表面抗体的乳胶颗粒被证明可用于检测人血样本中的乙肝表面抗原。在敏感性和检测速度方面，抗体包被乳胶凝集试验似乎优于传统免疫扩散技术。