Lewicki Z, Figurski R, Sulikowska A
Pol Med Sci Hist Bull (1973). 1975 Sep-Dec;15(5-6):539-49.
The dynamics of regeneration of small-intestine epithelium was studied in rats irradiated with X-rays in sublethal doses of 550, 600 or 750 R. Sixty-two irradiated and 22 control animals were used in the experiment. They were killed 1,2,4,6,8,14 and 25 days after the irradiation. Specimens of duodenum and jejunum were examined histologically, the sections being stained with H.E. and p.a.S. Already 1 and 2 days after irradiation the intestinal villi became shorter and deformed. The blood vessels were damaged, the enterocytes showed features of degeneration and vocuolization, the epithelium was detached by the exudate which accumulated in the stroma. Irradiation markedly disturbed the regeneration of intestinal epithelium in the period from the 1st to the 6th day. Cytological calculations indicate that on the 1st and 2nd days after irradiation the number of epithelial cells of the villi, and particularly of young cryptal ones, markedly dropped. On the 4th and 6th days increased proliferation of young cryptal cells considerably surpassed the physiological rate. The accompanying disturbances in differentiation consisted in a decreased acidophilic to basophilic cells ratio and in retardation of maturation of goblet cells. The absolute number of goblet cells was increased, as well as their proportion to the number of enterocytes.
研究了用550、600或750伦琴亚致死剂量X射线照射的大鼠小肠上皮的再生动力学。实验使用了62只受照射动物和22只对照动物。在照射后1、2、4、6、8、14和25天处死动物。对十二指肠和空肠标本进行组织学检查，切片用苏木精-伊红（H.E.）和过碘酸雪夫（p.a.S.）染色。照射后1天和2天，肠绒毛就变短并变形。血管受损，肠上皮细胞出现变性和空泡化特征，上皮被积聚在基质中的渗出物分离。在第1天至第6天期间，照射明显干扰了肠上皮的再生。细胞学计算表明，照射后第1天和第2天，绒毛上皮细胞，尤其是隐窝年轻细胞的数量明显下降。在第4天和第6天，隐窝年轻细胞增殖增加，大大超过生理速率。伴随的分化障碍表现为嗜酸性细胞与嗜碱性细胞比例降低以及杯状细胞成熟延迟。杯状细胞的绝对数量增加，其与肠上皮细胞数量的比例也增加。
