Estein Silvia M, Baldi Pablo C, Bowden Raúl A
Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, UNICEN, Tandil, Argentina.
J Vet Diagn Invest. 2002 Sep;14(5):407-11. doi: 10.1177/104063870201400508.
The aim of this work was to compare the performance of 6 serological tests using outer or internal antigens from Brucella for the diagnosis of Brucella ovis infection in sheep in an endemic area. Outer membrane antigens included a hot saline extract (HS) and the rough lipopolysaccharide (R-LPS) from B. ovis. Internal antigens were LPS-free total cytosolic proteins (CP) and an 18-kDa cytosolic protein (p18) from Brucella spp. Sera from 200 sheep from naturally infected flocks were assayed by agar gel immunodiffusion test (AGID) and by complement fixation test (CFT), both using HS, and by 4 ELISA using HS, R-LPS, CP, and p18, respectively. The percentage of positive results was 45.5% for ELISA with HS, 42.0% for ELISA with p18, 39.5% for CFT, 33.5% for ELISA with R-LPS, 29.0% for ELISA with CP, and 18.0% for AGID. Taking CFT as the reference test for calculating relative test parameters, the ELISA with HS had the best sensitivity (96.2%), while AGID and the ELISA with R-LPS had the best specificity (96.6%). The ELISA with CP was not more sensitive than the ELISA with p18 (67.1% vs. 79.7%) in spite of the higher number of antigens in CP. The lower relative sensitivity of tests using internal antigens might reflect a lack of antibodies to cytosolic proteins in some infected animals or a shorter persistence of these antibodies relative to antibodies to outer membrane components after recovery from infection.
本研究的目的是比较6种血清学检测方法的性能，这些方法使用布鲁氏菌的外抗原或内抗原，用于诊断地方流行区绵羊的绵羊布鲁氏菌感染。外膜抗原包括热盐水提取物（HS）和绵羊布鲁氏菌的粗糙脂多糖（R-LPS）。内抗原是布鲁氏菌属的无LPS总胞质蛋白（CP）和一种18 kDa胞质蛋白（p18）。对来自自然感染羊群的200只绵羊的血清进行琼脂凝胶免疫扩散试验（AGID）和补体结合试验（CFT）检测，二者均使用HS，同时分别使用HS、R-LPS、CP和p18进行4种ELISA检测。HS-ELISA的阳性结果百分比为45.5%，p18-ELISA为42.0%，CFT为39.5%，R-LPS-ELISA为33.5%，CP-ELISA为29.0% , AGID为18.0%。以CFT作为计算相对检测参数的参考检测方法，HS-ELISA具有最佳敏感性（96.2%），而AGID和R-LPS-ELISA具有最佳特异性（96.6%）。尽管CP中的抗原数量更多，但CP-ELISA并不比p18-ELISA更敏感（分别为67.1%和79.7%）。使用内抗原的检测方法相对敏感性较低，可能反映了一些感染动物缺乏针对胞质蛋白的抗体，或者相对于感染恢复后针对外膜成分的抗体，这些抗体的持续时间较短。
