Ficapal A, Alonso-Urmeneta B, Velasco J, Moriyón I, Blasco J M
Laboratori de Sanitat Ramadera, Lleida, Spain.
Vet Rec. 1995 Aug 5;137(6):145-7. doi: 10.1136/vr.137.6.145.
The complement fixation and gel diffusion tests for Brucella ovis ram epididymitis were compared with an indirect ELISA using antigens from B ovis extracted in hot saline, rough B ovis lipopolysaccharide or a cytosolic fraction of rough B melitensis 115, and commercial anti-IgG (heavy and light chain specificity) or protein G conjugates. None of the antigens and conjugates used in the ELISA gave better results in terms of sensitivity and specificity than the complement fixation or the gel diffusion tests with the sera of 41 rams naturally infected with B ovis, 17 rams inoculated conjunctivally with B ovis and 53 Brucella-free rams. The protein G conjugate significantly reduced the background reactivity of the sera from the Brucella-free rams but did not improve the sensitivity of the ELISA with the anti-IgG conjugate.
对绵羊种布鲁氏菌公羊附睾炎的补体结合试验和凝胶扩散试验，与使用在热盐水中提取的绵羊种布鲁氏菌抗原、粗糙型绵羊种布鲁氏菌脂多糖或粗糙型马尔他布鲁氏菌115胞质组分，以及商用抗IgG（重链和轻链特异性）或蛋白G缀合物的间接ELISA进行了比较。对于41只自然感染绵羊种布鲁氏菌的公羊、17只经结膜接种绵羊种布鲁氏菌的公羊和53只无布鲁氏菌公羊的血清，ELISA中使用的任何抗原和缀合物在敏感性和特异性方面都没有比补体结合试验或凝胶扩散试验取得更好的结果。蛋白G缀合物显著降低了无布鲁氏菌公羊血清的背景反应性，但并未提高抗IgG缀合物ELISA的敏感性。
