用于诊断公羊绵羊布鲁氏菌感染的酶联免疫吸附测定的诊断敏感性和特异性。
Diagnostic sensitivity and specificity of an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis infection in rams.
作者信息
Cho H J, Niilo L
出版信息
Can J Vet Res. 1987 Jan;51(1):99-103.
An enzyme-linked immunosorbent assay (ELISA) for antibody to Brucella ovis was compared with a standard complement fixation test. Sera of 176 rams from uninfected flocks gave 175 negative and one suspect ELISA reaction (diagnostic specificity 99.4%) whereas the complement fixation test yielded 167 negative, seven suspect and two anticomplementary reactions (diagnostic specificity of 96.0%). Diagnostic sensitivity was evaluated on sera of 79 rams from which B. ovis had been isolated. The ELISA showed 75 positive and four suspect reactions, while complement fixation test revealed 64 positive, 13 suspect and two negative results. Considering both positive and suspect reactions, the diagnostic sensitivity was 100% for ELISA and 97.5% for complement fixation test. The ELISA method was considered more specific, more sensitive and technically more advantageous than complement fixation test as a serodiagnostic test for B. ovis infection in rams.
对一种用于检测绵羊布鲁氏菌抗体的酶联免疫吸附测定（ELISA）与标准补体结合试验进行了比较。来自未感染羊群的176只公羊的血清，ELISA检测有175份呈阴性，1份呈可疑反应（诊断特异性为99.4%），而补体结合试验有167份呈阴性，7份呈可疑反应，2份呈抗补体反应（诊断特异性为96.0%）。对从79只公羊中分离出绵羊布鲁氏菌的血清进行了诊断敏感性评估。ELISA检测显示75份呈阳性，4份呈可疑反应，而补体结合试验显示64份呈阳性，13份呈可疑反应，2份呈阴性结果。综合阳性和可疑反应来看，ELISA的诊断敏感性为100%，补体结合试验为97.5%。作为公羊绵羊布鲁氏菌感染的血清学诊断试验，ELISA方法被认为比补体结合试验更具特异性、更敏感且在技术上更具优势。
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