Squirrell J, Hollingsworth P M, Bateman R M, Tebbitt M C, Hollingsworth M L
Royal Botanic Garden, 20 A Inverleith Row, Edinburgh EH3 5LR, UK.
Mol Ecol. 2002 Oct;11(10):1957-64. doi: 10.1046/j.1365-294x.2002.01610.x.
The genus Epipactis contains a problematical complex of autogamous taxa among which species limits are difficult to define. Different authors have treated these plants in different ways, some recognizing the different taxa as distinct species, others considering them as minor intraspecific variants. These contrasting treatments have a direct impact on the conservation resources and status such plants command; 'endemic orchid species' are perceived as having high conservation value, 'localized minor variants' are not. We used allozyme and chloroplast restriction fragment length polymorphism (RFLP) and sequencing analyses to investigate patterns of population genetic structure underlying the taxonomic complexity in this group. Populations of E. dunensis, E. leptochila and E. muelleri were homozygous and uniform for all loci studied here. There were, however, fixed genetic differences among these taxa. Comparisons with published data from the putative progenitor species for the autogamous taxa (the widespread, allogamous E. helleborine) suggest iterative origins of autogamy, rather than the self-pollinating taxa all being merely mutational variants of a single autogamous lineage.
火烧兰属包含一群自花授粉的分类群，其情况复杂，物种界限难以界定。不同的作者对这些植物有不同的处理方式，一些人将不同的分类群视为不同的物种，另一些人则将它们视为种内的微小变种。这些截然不同的处理方式直接影响到此类植物所获得的保护资源和地位；“地方性兰花物种”被视为具有很高的保护价值，而“局部微小变种”则不然。我们利用等位酶、叶绿体限制性片段长度多态性（RFLP）和测序分析，来研究该类群分类复杂性背后的种群遗传结构模式。在此研究的所有位点上，邓氏火烧兰、细距火烧兰和缪氏火烧兰的种群都是纯合且一致的。然而，这些分类群之间存在固定的遗传差异。与已发表的自花授粉分类群假定祖先物种（广泛分布的异花授粉植物欧洲火烧兰）的数据比较表明，自花授粉是多次起源的，而不是所有的自花授粉分类群仅仅是单一自花授粉谱系的突变变种。
