Takewaki K, Ohta Y
Endocrinol Jpn. 1975 Dec;22(6):561-5. doi: 10.1507/endocrj1954.22.561.
In Experiment 1, female rats were given a single subcutaneous injection of 1.25 mg 5alpha-dihydrotestosterone (DHT) or its propionate (DHTP) on day 5 of postnatal life. All of them showed regular estrous cycles as adults like untreated control animals. At about 60 days of age, the rats were ovariectomized and given 7 daily injections of 2 mg progesterone (P) plus 0.2 mug estradiol-17beta (ED). Uterine trauma applied on the 4th day of P-ED injections resulted in well developed deciduomata in all animals by the day after the last injection. This made a sharp contrast to the failure of female rats receiving testosterone propionate (TP) neonatally to give a positive response under similar experimental conditions (Takewaki and Ohta, 1974). The mean weight of traumatized horns was significantly larger in DHTP-treated rats (but not in DHT-treated rats) than in controls. In Experiment 2, rats were ovariectomized on day 4 and given a dose of 1.25 mg DHT or DHTP on day 5. Controls were ovariectomized on day 4 but not given any steroid on the next day. A series of 7 daily injections of 2 mg P plus 0.2 mug ED was started at about 60 days of age, after the animals had received 3 daily injections of 0.2 mug ED or 30 daily injections of 0.1 mug ED. Incidence of deciduomata following uterine traumatization was markedly lowered only in animals treated with DHTP neonatally and given 0.1 mug ED for 30 days as adults, no significant differences being found in both incidence and size of deciduomata among the other groups. It was suggested that the effects of neonatal steroid administration on uterine responsiveness in adulthood are specific to the steroid. The previous conclusion that persistent estrus in androgen-sterilized rats plays a part in the reduction of uterine responsiveness was confirmed. An exposure of rats to estrogen for a prolonged postpuberal period was without effect, unless the animals had received enough androgen neonatally.
在实验1中，雌性大鼠在出生后第5天接受一次皮下注射1.25毫克5α-二氢睾酮（DHT）或其丙酸酯（DHTP）。所有这些大鼠成年后都表现出规律的发情周期，如同未处理的对照动物一样。在大约60日龄时，将大鼠卵巢切除，并每日注射7次2毫克孕酮（P）加0.2微克雌二醇-17β（ED）。在注射P-ED的第4天施加子宫创伤，到最后一次注射后的第二天，所有动物都形成了发育良好的蜕膜。这与新生期接受丙酸睾酮（TP）的雌性大鼠在类似实验条件下未能产生阳性反应形成了鲜明对比（武aki和太田，1974年）。DHTP处理的大鼠（但不是DHT处理的大鼠）创伤角的平均重量显著大于对照组。在实验2中，大鼠在第4天卵巢切除，并在第5天给予1.25毫克DHT或DHTP剂量。对照组在第4天卵巢切除，但次日未给予任何类固醇。在动物接受3次每日0.2微克ED注射或30次每日0.1微克ED注射后，大约60日龄时开始一系列每日7次2毫克P加0.2微克ED的注射。仅在新生期用DHTP处理且成年后给予0.1微克ED 30天的动物中，子宫创伤后蜕膜的发生率显著降低，其他组在蜕膜的发生率和大小方面均未发现显著差异。提示新生期给予类固醇对成年期子宫反应性的影响是类固醇特异性的。先前关于雄激素绝育大鼠持续发情在子宫反应性降低中起作用的结论得到了证实。大鼠在青春期后长期暴露于雌激素没有效果，除非动物在新生期接受了足够的雄激素。
