Mitschek G H
Exp Pathol (Jena). 1975;10(3-4):156-66.
In earlier studies [see literature (2, 20, 21, 22)] observations concerning the effect of Solanum Melongena (Sol. Mel.) (violet egg plants) on experimentally induced artheromatosis in rabbits were reported. This study aims to investigate histologically the effect of Sol. Mel. on experimental atheromatosis in mean-term (2--4 weeks) and long-term (8--12 weeks) tests.
For details of the test arrangement see AUBOCK and MITSCHEK, Exp. Path. 9, 323--335 (1974). From one portion of the material for histological examination cryostat sections were prepared, the other portion was fixed in Bouin's solution or in Baker's formol and paraffin-embedded at 58--60 degrees C (cross sections of the aorta). The specimens were stained as follows: erythrosin in conjunction with haematoxylin; aqueous or alcoholic solutions of toluidine blue (pH 3.5 to 5, and 7); methylene blue (pH 2.5 to 5, and 8); alcian blue (pH 2.5); Schiff-PAS; alcian blue and PAS in combination; Sudan III; elastica-van Gieson (blue) and Weigert's iron haematoxylin. For demonstration of mucopolysaccharides (MPS) the control sections were pretreated in a 10% Takadiastase solution for 10 minutes at 37 degrees C. In liver sections PAS-reactivity was additionally blocked by dimedone (cyclohexanedione) for elective demonstration of glycogen. The tissue sections were studied in transmission--, incident- and dark field illumination.
lipid deposits as demonstrated by surface preparation technique could not be seen in paraffin sections after just one day. In the vascular wall histological changes were earliest visible after 10 to 14 days (enlargement of the subendothelial space and honeycombed edema with fine dispersed lipids). At this stage a "haematoxylin-effect" did yet not develop. Sometimes these alterations were also present in the upper layers of the media. They always first occurred in the aortic arch. At this time the Sol. Mel.-treated animals of group II only occasionally developed superficial edemas. Enlargement of the media with edematous infiltration and loosening of the elastic fibers was similar in both cases (figs. 1a and 1b). Fatty degeneration of the liver was already macroscopically visible on day 14; in untreated animals of group I it was more expressive than in group II (figs. 2a and 2b) -- this likewise applied to the cholesterol content (figs. 3a and 3b). After about 30 days in group I the earliest macroscopically visible plaques occurred prevailingly in the aortic arch and in the thoracic aorta. The development of such small foam cell plaques could be continuously observed with a hand lens (fig. 4a). In group II, however, it was not possible to observe any development of plaques with a hand lens, persistence of edemas was demonstrable with such (fig. 4b). Within these, fine dispersed droplets were present (fig. 5) but exact localization was only possible by electron microscopy [see literature(2)].
在早期研究中[见文献(2, 20, 21, 22)]，曾报道过有关茄子对家兔实验性动脉粥样硬化影响的观察结果。本研究旨在通过组织学方法，在中期（2 - 4周）和长期（8 - 12周）实验中，研究茄子对实验性动脉粥样硬化的影响。
实验安排的详细内容见奥博克和米切克所著《实验病理学》9, 323 - 335(1974)。从用于组织学检查的材料中，一部分制成低温恒温器切片，另一部分固定于布安氏溶液或贝克氏甲醛溶液中，并在58 - 60摄氏度下石蜡包埋（主动脉横断面）。标本按如下方法染色：伊红与苏木精联合染色；甲苯胺蓝水溶液或酒精溶液（pH值为3.5至5以及7）；亚甲蓝（pH值为2.5至5以及8）；阿尔辛蓝（pH值为2.5）；席夫 - PAS；阿尔辛蓝与PAS联合染色；苏丹III；弹性纤维 - 范吉森（蓝色）染色及魏格特氏铁苏木精染色。为显示粘多糖（MPS），对照切片在37摄氏度下于10%胰淀粉酶溶液中预处理10分钟。在肝脏切片中，为选择性显示糖原，二甲基酮（环己二酮）可额外阻断PAS反应性。组织切片在透射光、落射光和暗场照明下进行研究。
仅一天后，石蜡切片中通过表面制备技术所显示的脂质沉积就无法看到。在血管壁中，组织学变化最早在10至14天后可见（内皮下间隙增大以及伴有细微分散脂质的蜂窝状水肿）。在此阶段，“苏木精效应”尚未出现。有时这些改变也出现在中膜上层。它们总是首先出现在主动脉弓。此时，II组经茄子处理的动物仅偶尔出现表面水肿。中膜增大伴有水肿性浸润以及弹性纤维松弛在两种情况下相似（图1a和1b）。肝脏的脂肪变性在第14天肉眼已可见；I组未处理动物的脂肪变性比II组更明显（图2a和2b）——胆固醇含量情况同样如此（图3a和3b）。I组大约30天后，最早肉眼可见的斑块主要出现在主动脉弓和胸主动脉。用放大镜可连续观察到此类小泡沫细胞斑块的形成（图4a）。然而，在II组中，用放大镜无法观察到任何斑块形成，仅可证明水肿持续存在（图4b）。在这些水肿内，存在细微分散的液滴（图5），但只有通过电子显微镜才能准确定位[见文献(2)]。
