Mitschek G H
Exp Pathol (Jena). 1975;10(3-4):167-79.
The mode of action of Solanum Melongena (Sol. Mel.) in its inhibitory effect on the development of atheromatous plaques in cholesterol-induced atheromatosis in rabbits is still unexplained. In this paper the author reports his enzyme histochemical, physiopathological and chemical observations drawing conclusions for future experiments.
Animals and experimental conditions were the same as described in the recent studies (see literature no. 2, 15, 16, 17, 18). 1. Enzyme histochemistry: The aortas were removed immediately after death, placed in Holt's solution, freed from the advential fatty tissue, cut into 5-6 mm high cylinders and either processed instantly or preserved in the same fluid at +2 degrees C. From the individual portions cryostat sections (10 mum thick) were cut after the method of Pearse. They were mounted to cover glasses (22 X22) and placed in substrate-filled specially produced cuvettes. The capacity of such a cuvette was 10 ml with room for 4 cover glasses each 4 mm apart. For protection against vaporization small caps of plastic foil were used. The method given by GLOCKNER and SCHMIDT (7) who recently reported their studies in the rat aorta, yielded satisfactory results in small arteries. In thereabbit aorta, however, the results varied markedly, which may be due to greater thickness of the vascular wall. Consequently, despite of all its advantages, this technique could not be employed in this study. The following enzymes were demonstrated: hydrolases: phosphomonoesterase I--3.1.3.1. with naphthol AS-sulphate after BURSTONE in the modification of LOJDA phosphomonoesterase II--3.1.3.2. after BURSTONE as modified by LOJDA with naphthol AS-phosphate and hexazonium pararosanilin leucine aminopeptidase--3.4.1.1. technique according to NACHLAS et al. with leucine beta-naphthylamide lipase--3.1.1.3. after GOMORI (Tween 80) adenosine-5-phosphatase (5'nucleotidase)--3.1.3.5. after GOMORI succinic dehydrogenase--1.3.99.1.--method according to NACHLAS et al. NAD (NADH and NADP (NADPH) diaphorase--1.4.1.2.--1.4.1.4., technique of SCARPELLI, HESS and PEARSE as modified by LOJDA (see article) Substratefree or heatinactivated controls were prepared for each histochemical demonstration. Several additional series with phenazine methosulphate (PMS) as an intermediate electron acceptor or such without PMS as well as preparations with KCN or without KCN were examined for the demonstration of anaerobic dehydrogenases. However, to avoid the nothing dehydrogenase effect only PMS-processed specimens were evaluated. For histological information several additional sections of the tissue samples were stained with haematoxylin-eosin or with elastica-van Gieson and Weigert's iron haematoxylin in combination. The enzyme histochemical preparations were embedded in Kaiser's glycerine gelatin, the histological sections were embedded in Caedox. The test animals were grouped like in the recent experiments (see literature no. 2, 15, 16, 17, 18). 2...
茄子（Solanum Melongena，简称Sol. Mel.）对家兔胆固醇诱导的动脉粥样硬化中动脉粥样斑块形成的抑制作用机制仍未明确。在本文中，作者报告了其酶组织化学、生理病理学及化学观察结果，并为未来实验得出结论。
动物及实验条件与近期研究中所述相同（见参考文献2、15、16、17、18）。1. 酶组织化学：动物死后立即取出主动脉，置于霍尔特氏溶液中，去除外膜脂肪组织，切成5 - 6毫米高的圆柱体，立即进行处理或保存在+2℃的同一溶液中。按照皮尔斯的方法，从各个部分切取低温恒温切片（10微米厚）。将切片安装在盖玻片（22×22）上，放入特制的装有底物的比色皿中。这种比色皿的容量为10毫升，可容纳4个盖玻片，每个盖玻片间距4毫米。为防止蒸发，使用塑料箔小帽。格洛克纳和施密特（7）最近在大鼠主动脉研究中给出的方法，在小动脉中取得了满意结果。然而，在兔主动脉中，结果差异显著，这可能是由于血管壁更厚所致。因此，尽管该技术有诸多优点，但本研究中无法采用。检测了以下几种酶：水解酶：磷酸单酯酶I - 3.1.3.1.，采用经洛伊达改良的伯斯顿法，以萘酚AS - 硫酸盐为底物；磷酸单酯酶II - 3.1.3.2.，采用经洛伊达改良的伯斯顿法，以萘酚AS - 磷酸盐和对品红六偶氮为底物；亮氨酸氨肽酶 - 3.4.1.1.，按照纳赫拉斯等人的技术，以亮氨酸β - 萘酰胺为底物；脂肪酶 - 3.1.1.3.，采用戈莫里法（吐温80）；腺苷 - 5 - 磷酸酶（5'核苷酸酶） - 3.1.3.5.，采用戈莫里法；琥珀酸脱氢酶 - 1.3.99.1.，按照纳赫拉斯等人的方法；NAD（NADH）和NADP（NADPH）黄递酶 - 1.4.1.2. - 1.4.1.4.，采用经洛伊达改良的斯卡佩利、赫斯和皮尔斯技术（见文章）。每次组织化学检测均制备无底物或热灭活对照。为检测厌氧脱氢酶，还检查了几个额外系列，其中包括以吩嗪硫酸甲酯（PMS）作为中间电子受体或不含PMS的系列，以及含KCN或不含KCN的制剂。然而，为避免无脱氢酶效应，仅评估经PMS处理的标本。为获取组织学信息，组织样本的几个额外切片用苏木精 - 伊红染色，或与弹性纤维 - 范吉森染色以及魏格特铁苏木精联合染色。酶组织化学制剂用凯泽甘油明胶包埋，组织学切片用卡多克斯包埋。实验动物分组与近期实验相同（见参考文献2、15、16、17、18）。2...
