[实验性异种免疫性胰腺炎。1. 用抗小鼠胰腺兔血清处理后小鼠外分泌胰腺的组织学发现（作者译）]

[Experimental xenogenic immune pancreatitis. 1. Histological findings in the mouse exocrine pancreas after treatment with anti-mouse pancreas rabbit serum (author's transl)].

作者信息

Nizze H

出版信息

Exp Pathol (Jena). 1975;11(1-2):10-20.

Abstract

QUESTION

In the pathogenesis of some forms of chronic pancreatitis an immunological influence has been discussed. Therefore, experimental studies of the exocrine pancreas after immunological damage should be done. In this communication histological findings on the mouse exocrine pancreas are presented after treatment with anti-mouse pancreas rabbit serum.

MATERIAL AND METHODS

a total of 71 adult male white mice was divided into 4 groups treated in the following way: 1. 35 animals with anti-mouse pancreas rabbit serum 2. 14 animals with normal rabbit serum 3. 16 animals with physiological saline 4. 6 animals remained untreated. The sera under study and the saline were, without exception, administered intraperitoneally with single doses of 0.3 ml once, resp. twice daily for 0 up to 16 days. Constantly, 3 hours before sacrifice, the animals received the last injection. For histological examination, the fresh pancreas was fixed in formaline. After embedding in paraffin, sections were stained by the following methods: hematoxyline-eosin, trichrome according to Goldner, Giemsa staining, PAS-reaction, and tryptophane demonstration according to ADAMS (1957).

摘要

问题

在某些形式的慢性胰腺炎发病机制中，免疫影响已被讨论。因此，应该进行免疫损伤后外分泌胰腺的实验研究。在本报告中，呈现了用抗小鼠胰腺兔血清处理后小鼠外分泌胰腺的组织学发现。

材料与方法

总共71只成年雄性白色小鼠被分为4组，按以下方式处理：1. 35只动物注射抗小鼠胰腺兔血清；2. 14只动物注射正常兔血清；3. 16只动物注射生理盐水；4. 6只动物未处理。所研究的血清和生理盐水无一例外均通过腹腔注射，单剂量为0.3 ml，分别每日注射1次或2次，持续0至16天。在处死动物前3小时，持续给动物进行最后一次注射。为进行组织学检查，将新鲜胰腺固定在福尔马林中。石蜡包埋后，切片用以下方法染色：苏木精 - 伊红染色、根据戈德纳法进行的三色染色、吉姆萨染色、PAS反应以及根据亚当斯（1957年）法进行的色氨酸显示。