Green E, Reible S
Proc Natl Acad Sci U S A. 1975 Jan;72(1):253-7. doi: 10.1073/pnas.72.1.253.
The thesis is developed that an acceptable model of biological energy coupling must have universal application. The paired moving charge model of mitochondrial energy coupling is examined from the standpoint of this thesis. Fundamental to this model is the notion that energy coupling involves interaction between paired uncompensated charged species in two vectorially aligned and spatially separated reaction centers. The two charge-separating devices are assumed to be the electron transfer chain (in chloroplast and mitochondria) and intrinsic ionophores (in all transducing organelles and kinases). The universality of the ionophore principle becomes then the crucial test of the validity of the paired moving charge model. The multiple facets of ionophore-mediated couples processes are explored, e.g., coupled hydrolysis of ATP, hormonal control of ion movements, and active transport.
本文提出一个观点，即可接受的生物能量耦合模型必须具有普遍适用性。从这一观点出发，对线粒体能量耦合的成对移动电荷模型进行了研究。该模型的基础理念是，能量耦合涉及两个在矢量方向上对齐且空间上分离的反应中心中，成对的未补偿带电物种之间的相互作用。假定两个电荷分离装置分别是电子传递链（存在于叶绿体和线粒体中）和内在离子载体（存在于所有转导细胞器和激酶中）。那么离子载体原理的普遍性就成为了成对移动电荷模型有效性的关键检验。本文探讨了离子载体介导的耦合过程的多个方面，例如ATP的耦合水解、离子运动的激素控制以及主动运输。
