Powers M B, Coate W B, Lewis T R
Arch Environ Health. 1975 Apr;30(4):165-7. doi: 10.1080/00039896.1975.10666668.
In a study to evaluate its acnegenic potential, increasing concentrations of 1,2,4-trichlorobenzene were applied topically to the ventral surface of the rabbit ear three times weekly for 13 weeks. Additional groups of rabbits received similar treatment with petroleum ether (solvent controls), received no treatment (negative controls), and received four once-weekly treatments with hexachlorodiphenyloxide, a known chloracnegenic agent (positive controls). Skin response to 1,2,4-trichlorobenzene was characterized grossly by dermal irritation directly related to the concentration of test material; there were the associated histologic changes of acanthosis and hyperkeratosis; there was no primary follicular involvement characteristic of acneform dermatitis. Dermal responses to hexachlorodiphenyloxide consisted of gross follicular enlargement, with waxy excretion on pressure, and severe scaling. The affected ear appeared thickened up to three times normal size and histologic sections showed primary follicular involvement characterized by marked thickening of the sheath and marked distention of the follicles with keratin, with resultant comedone formation, typical of chloracne.
在一项评估其致粉刺潜力的研究中，每周三次在兔耳腹面局部涂抹浓度递增的1,2,4-三氯苯，持续13周。另外几组兔子分别接受用石油醚进行的类似处理（溶剂对照）、不接受处理（阴性对照）以及接受已知致氯痤疮剂六氯二苯醚的每周一次共四次处理（阳性对照）。1,2,4-三氯苯引起的皮肤反应总体表现为与受试物浓度直接相关的皮肤刺激；伴有棘皮症和角化过度的相关组织学变化；不存在痤疮样皮炎特有的原发性毛囊受累情况。六氯二苯醚引起的皮肤反应包括肉眼可见的毛囊肿大，按压时有蜡样排泄物，以及严重脱屑。受影响的耳朵增厚至正常大小的三倍，组织学切片显示原发性毛囊受累，其特征为鞘显著增厚，毛囊因角质而显著扩张，导致形成黑头粉刺，这是氯痤疮的典型表现。
