Kligman A M, Kwong T
Br J Dermatol. 1979 Jun;100(6):699-702. doi: 10.1111/j.1365-2133.1979.tb08075.x.
A simplified comedogenic assay is described in which test materials are applied for 2 weeks to the ears of rabbits just external to the ear canal. Excised tissue is thus immersed in water at 60 degrees C for 2 min, yielding a sheet of epidermis with microcomedones attached. The magnitude of follicular hyperkeratosis is extracted with the stereomicroscope. Sixteen materials were evaluated by the new and old model which required histological sectioning. Agreement was excellent.
描述了一种简化的致粉刺试验，其中将测试材料应用于兔耳道外侧的耳朵两周。切除的组织因此在60摄氏度的水中浸泡2分钟，得到附着有微粉刺的表皮片。用体视显微镜提取毛囊角化过度的程度。通过需要组织切片的新旧模型对16种材料进行了评估。一致性非常好。
