Motoyoshi K
Br J Dermatol. 1983 May;108(5):573-9. doi: 10.1111/j.1365-2133.1983.tb01059.x.
Three concentrations of tetradecane and liquid paraffin were applied daily for 4 weeks to the external ear skin of rabbits, and the average diameter of the follicular lesions was measured under the Leitz MZ surface microscope. These observations showed that the increase in the size of follicular lesions with tetradecane was dose-dependent and the maximum diameter was reached on the 14th day. Serial biopsies were also taken for routine histology, and undiluted tetradecane was shown to produce follicular hyperkeratosis, microcomedones, closed comedones and open comedones. A good correlation was obtained between the surface observations and the histological findings. The surface microscope provides a simple and convenient in vivo method for evaluating comedo formation.
每天将三种浓度的十四烷和液体石蜡涂抹于家兔外耳道皮肤，持续4周，然后在Leitz MZ体视显微镜下测量毛囊病变的平均直径。这些观察结果表明，十四烷导致的毛囊病变大小增加呈剂量依赖性，在第14天达到最大直径。还进行了系列活检以进行常规组织学检查，结果显示未稀释的十四烷可导致毛囊角化过度、微粉刺、闭合性粉刺和开放性粉刺。表面观察结果与组织学发现之间具有良好的相关性。体视显微镜为评估粉刺形成提供了一种简单便捷的体内方法。
