Ruitton A, Frederich F
Diabete Metab. 1975 Sep;1(3):143-50.
Rabbits were immunized with polymerized glucagon and specific antisera obtained. Antiserum used at a dilution of 1/24 000 (final concentration) exhibited a cross reaction of 1,6% with enteroglucagon. Radioimmunoassay of serum glucagon employed dextran coated charcoal and 125 I glucagon. Mean concentration in normal fasting children was 153 pg/ml +/- 27 (S.E.M.) and the molar ratio of insulin/glucagon was between 1,9 and 3,9. In the case of premature, dysmature and diabetic children or children from diabetic mothers, the levels did not differ significantly from normal. Results of glucagon secretion stimulation tests using arginine insulin combinations are also reported.
用聚合胰高血糖素对兔子进行免疫并获得特异性抗血清。使用稀释度为1/24000（终浓度）的抗血清与肠胰高血糖素的交叉反应率为1.6%。血清胰高血糖素的放射免疫测定采用葡聚糖包被活性炭和125I胰高血糖素。正常空腹儿童的平均浓度为153 pg/ml +/- 27（标准误），胰岛素/胰高血糖素的摩尔比在1.9至3.9之间。对于早产儿、发育不成熟儿、糖尿病儿童或患有糖尿病母亲的儿童，其水平与正常儿童无显著差异。还报告了使用精氨酸胰岛素组合进行胰高血糖素分泌刺激试验的结果。
