Ioachim H L, Dorsett B H, Sabbath M, Barber H R
Natl Cancer Inst Monogr. 1975 Oct;42:45-62.
The ultrastructure of the major histologic types of ovarian carcinoma was investigated as part of a multilateral study of this tumor. The nuclear and nucleolar changes in size, shape, and structure correlated well with the degree of malignancy and tumor grading. Cytoplasmic organelles and intercellular junctions were abundant and fairly well differentiated even in ovarian carcinomas of higher grade and stage. Active processes of synthesis and secretion taking place in most of these tumors were suggested by the presence of a richly granulated endoplasmic reticulum, dilated cisternae, and numerous secretory granules. Seventy-eight different ovarian carcinomas of all histologic types were cultured in vitro for periods of up to 300 days, and their morphology in light and electron microscopy was compared to that of the original tumors. The cultures displayed a consistent pattern of growth which led to the conclusion that ovarian cancer cells in vitro preserve their salient features and are representative of the tumors of origin. Heterologous antisera raised with pooled extracts of various types of ovarian carcinomas reacted specifically in immunodiffusion and immunofluorescence tests only with ovarian carcinomas and not with normal ovaries, benigh ovarian tumors, and nonovarian malignant neoplasms, indicating the presence of a cross-reacting specific antigen for ovarian carcinomas. In other studies, autologous antibodies were isolated from antigen-antibody complexes recovered from peritoneal effusions of patients with ovarian carcinomas. These antibodies displayed a high degree of specificity against ovarian carcinoma cells when tested in immunofluorescence assays.
作为对该肿瘤多边研究的一部分，对卵巢癌主要组织学类型的超微结构进行了研究。细胞核和核仁在大小、形状和结构上的变化与恶性程度和肿瘤分级密切相关。即使在高级别和晚期卵巢癌中，细胞质细胞器和细胞间连接也很丰富且分化良好。丰富的粗面内质网、扩张的潴泡和大量分泌颗粒的存在表明，这些肿瘤中的大多数都发生了活跃的合成和分泌过程。对78种所有组织学类型的不同卵巢癌进行了长达300天的体外培养，并将其光镜和电镜下的形态与原发肿瘤进行了比较。培养物呈现出一致的生长模式，由此得出结论，体外培养的卵巢癌细胞保留了其显著特征，代表了原发肿瘤。用各种类型卵巢癌的混合提取物制备的异种抗血清，在免疫扩散和免疫荧光试验中仅与卵巢癌发生特异性反应，而不与正常卵巢、良性卵巢肿瘤和非卵巢恶性肿瘤发生反应，这表明存在一种卵巢癌交叉反应特异性抗原。在其他研究中，从卵巢癌患者腹腔积液中回收的抗原-抗体复合物中分离出了自体抗体。这些抗体在免疫荧光试验中对卵巢癌细胞表现出高度特异性。
