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小型猪主动脉内皮细胞的体外培养与鉴定

In vitro cultivation and identification of aortic endothelium from miniature pig.

作者信息

Kerënyi T, Koch A S, Jellinek H, Csonka E

出版信息

Paroi Arterielle. 1975 Sep;3(1):31-41.

PMID:1234644
Abstract

Pieces of miniature pig aorta were explanted endothelial face down directly on the bottom of Petri dishes. On removal of the aorta fragments after 2-4 days of incubation single cells or small groups of viable endothelium were found attached. The cells developed into a permanent line (at present over 18 passages) and were identified as pure endothelial cells both by light- and electron microscopic examination.

摘要

将微型猪主动脉片内皮面朝下直接放置在培养皿底部。培养2 - 4天后取出主动脉碎片时,发现有单个细胞或小群存活的内皮细胞附着。这些细胞形成了一个永久细胞系(目前已传代超过18次),并且通过光镜和电镜检查均被鉴定为纯内皮细胞。

相似文献

1
In vitro cultivation and identification of aortic endothelium from miniature pig.小型猪主动脉内皮细胞的体外培养与鉴定
Paroi Arterielle. 1975 Sep;3(1):31-41.
2
[In vitro cultivation and identification of aortic endothelial cells].[主动脉内皮细胞的体外培养与鉴定]
Morphol Igazsagugyi Orv Sz. 1974 Jul;14(3):161-9.
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Establishment and characterization of endothelial cell lines from the aorta of miniature pig for the study of xenotransplantation.用于异种移植研究的小型猪主动脉内皮细胞系的建立与鉴定
Cell Biol Int. 2005 Aug;29(8):638-46. doi: 10.1016/j.cellbi.2005.03.016.
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Aortic endothelial and smooth muscle cell interactions in culture.培养条件下主动脉内皮细胞与平滑肌细胞的相互作用
Acta Morphol Hung. 1986;34(3):157-61.
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Isolation of rat aortic endothelial cells by primary explant techniques and their phenotypic modulation by defined substrata.通过原代外植体技术分离大鼠主动脉内皮细胞及其在特定基质上的表型调节。
Lab Invest. 1987 Jul;57(1):94-105.
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Endothelial glycosaminoglycans-in vitro studies.内皮糖胺聚糖——体外研究
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Lining of viable and nonviable allogeneic and xenogeneic cardiovascular tissue with cultured adult human venous endothelium.用培养的成人人类静脉内皮细胞覆盖有活力和无活力的同种异体及异种心血管组织。
J Thorac Cardiovasc Surg. 1993 Sep;106(3):434-43.
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A method to measure the pinocytic uptake of 125I-labelled polyvinylpyrrolidone by pig aortic smooth muscle and endothelial cells in culture.一种测量培养的猪主动脉平滑肌细胞和内皮细胞对¹²⁵I标记的聚乙烯吡咯烷酮的胞饮摄取的方法。
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The membrane potential of pig aortic endothelial cells.猪主动脉内皮细胞的膜电位
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引用本文的文献

1
Cultivation of microvascular endothelial cells from human preputial skin.
In Vitro. 1980 Aug;16(8):675-84. doi: 10.1007/BF02619197.
2
Interaction of plasmin with endothelial cells.纤溶酶与内皮细胞的相互作用。
Biochem J. 1984 Feb 15;218(1):119-24. doi: 10.1042/bj2180119.