Endothelial glycosaminoglycans-in vitro studies.

Endothelial cells were grown from pig aortic intimal explants. Collagen and glycosaminoglycan (GAG) content and GAG distribution was measured in the cells and in the culture medium as a function of the time on incubation and of subcultivation. The influence of the addition of chondroitin-6-sulfate or hyaluronic acid to the medium has also been studied.