The membrane potential of pig aortic endothelial cells.

Membrane potential and cell number of endothelial cells from pig aorta (line BSEz-3) were determined in vitro in different phases of growth. We found different membrane potentials for mononuclear (-8.6 mV) and multinuclear (-21.9 mV) endothelial cells. In both cases the maximum membrane potential occurred on the 6th day after seeding (during the exponential growth phase). The fraction of multinuclear endothelial cells was about 2.1% in the total culture. The membrane potentials of pig aortic endothelial cells are similar to our earlier values obtained from calf aortic endothelial cells.