Foussereau J, Herrmann B, Grosshans E, Petitjean J, Maleville J
Contact Dermatitis. 1975 Aug;1(4):200-6. doi: 10.1111/j.1600-0536.1975.tb05382.x.
Experimental sensitization to dinitro-2,4-chlorobenzene (DNCB) is being studied according to different techniques, the variable factors being the concentrations of DNCB, the number of applications and the time between the sensitizing application and the reading of the challenge test. The authors relate and discuss their technique (only one touch of a drop of 10% DNCB in acetone, covered with a cover-slip and left to evaporate), the results and the advantages: in particular, stanardization of the parameters of sensitization, (quantity of DNCB, area and time of application) and reduction in the risk of toxic effects inherent in excessively high concentrations of DNCB. In addition, the authors emphasize the fundamental significance of the length of time needed to acquire experimental sensitization.
正在根据不同技术研究对二硝基 - 2,4 - 氯苯（DNCB）的实验性致敏作用，可变因素包括DNCB的浓度、涂抹次数以及致敏涂抹与激发试验读数之间的时间间隔。作者阐述并讨论了他们的技术（仅用一滴10%的DNCB丙酮溶液轻触，盖上盖玻片并让其蒸发）、结果及优势：特别是致敏参数的标准化（DNCB的量、涂抹面积和时间）以及降低过高浓度DNCB固有的毒性作用风险。此外，作者强调了获得实验性致敏所需时间长度的根本重要性。
