Rees J L, Friedmann P S, Matthews J N
University Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, U.K.
Br J Dermatol. 1990 Jan;122(1):29-31. doi: 10.1111/j.1365-2133.1990.tb08236.x.
We have investigated the effect on sensitization of altering the area of application of 2,4,dinitrochlorobenzene (DNCB) at a constant dose per unit area. We showed that, when an area of less than 1 cm2 is used, this area is critical in determining the degree of sensitization. This contrasts with previous work that showed, for larger areas, an alteration in the area of application had little effect on sensitization, whereas keeping the area constant and increasing the concentration of DNCB increased the degree of sensitization. We suggest that not only is the amount of antigen important in determining response, but also the distribution of the antigen as presented to the afferent limb of the immune system.
我们研究了在每单位面积剂量恒定的情况下，改变2,4-二硝基氯苯（DNCB）的涂抹面积对致敏作用的影响。我们发现，当使用面积小于1平方厘米时，该面积对于确定致敏程度至关重要。这与之前的研究形成对比，之前的研究表明，对于较大面积，涂抹面积的改变对致敏作用影响不大，而保持面积不变并增加DNCB的浓度则会增加致敏程度。我们认为，不仅抗原的量在决定反应中很重要，而且呈现给免疫系统传入分支的抗原分布也很重要。
