Fagoaga Omar R, Nehlsen-Cannarella Sandra L
Department of Pathology, Immunology Center, Loma Linda University School of Medicine and Medical Center, CA 92354-2870, USA.
Dev Immunol. 2002 Mar;9(1):9-17. doi: 10.1080/10446670290030972.
Changes in programming of neonatal immune development were effected through maternal immune modulation (Leishmania major inoculation). In progeny of these dams, immune profiles in both blood and spleen were changed throughout the neonatal period and were pronounced after weaning. White blood cell (WBC) and lymphocyte counts in blood of 45-day-old progeny were two-fold less than control animals. In blood, proportions of B cells were greater, while T helpers, Tc/s and NK cells were less than in controls. In contrast, proportions of splenic B and NK cells were greater than controls. But, proportions of all T and Tc/s cells on d20 and 45 were lower than controls. In blood, absolute numbers of all T, Th naïve and Th memory cells were lower than in controls. In contrast, in the spleen, numbers of NK, T and Th naive and memory cells were up to 200% greater than in control pups. Cytokine responses of splenic lymphocytes stimulated through CD3 ligation revealed no difference in IL-4 production. In contrast, IL-2 and IFNgamma were lower on d45 and 5, respectively, in the experimental compared to control mice. These data support the hypothesis that maternal immune events during gestation can modulate the pattern of immune development in offspring.
通过母体免疫调节（接种硕大利什曼原虫）实现了新生儿免疫发育编程的改变。在这些母鼠的后代中，血液和脾脏中的免疫谱在整个新生儿期都发生了变化，断奶后更为明显。45日龄后代血液中的白细胞（WBC）和淋巴细胞计数比对照动物少两倍。在血液中，B细胞的比例更高，而辅助性T细胞、细胞毒性T细胞/杀伤性T细胞和自然杀伤细胞比对照组少。相比之下，脾脏中B细胞和自然杀伤细胞的比例高于对照组。但是，在第20天和第45天，所有T细胞和细胞毒性T细胞/杀伤性T细胞的比例均低于对照组。在血液中，所有T细胞、初始辅助性T细胞和记忆辅助性T细胞的绝对数量均低于对照组。相比之下，在脾脏中，自然杀伤细胞、T细胞以及初始和记忆辅助性T细胞的数量比对照幼崽高出200%。通过CD3连接刺激脾脏淋巴细胞产生的细胞因子反应显示，白细胞介素-4的产生没有差异。相比之下，与对照小鼠相比，实验小鼠在第45天和第5天的白细胞介素-2和干扰素-γ水平分别较低。这些数据支持以下假设：妊娠期间的母体免疫事件可调节后代的免疫发育模式。
