Hemsley S, Bailey G, Canfield P
Faculty of Veterinary Science B01, University of Sydney, NSW, Australia.
J Comp Pathol. 2002 Jul;127(1):69-71. doi: 10.1053/jcpa.2002.0555.
Species cross-reactive anti-peptide antibodies were assessed in formalin-fixed tissue for use in immunophenotyping of lymphosarcoma in two alpacas. Diagnosis of lymphosarcoma was made by routine histopathological examination. Primary antibodies used for immunophenotyping were anti-human CD3 and anti-human CD5 for T cells; and anti-human CD79a and anti-human CD79b for B cells/plasma cells. In one case, most of the neoplastic cells were labelled with both anti-CD3 and anti-CD79b, and smaller numbers were labelled with anti-CD79a. The other case was classified as a B-cell tumour on the basis of labelling of the majority of neoplastic cells with anti-CD79b and anti-CD79a. This is the first recorded attempt at immunophenotyping lymphosarcoma in alpacas and, to our knowledge, the first record of presumptive co-expression of T- and B-cell-associated molecules in lymphosarcoma in the veterinary literature.
在福尔马林固定组织中评估了种属交叉反应性抗肽抗体，用于两只羊驼淋巴瘤的免疫表型分析。淋巴瘤的诊断通过常规组织病理学检查进行。用于免疫表型分析的一抗包括针对T细胞的抗人CD3和抗人CD5；以及针对B细胞/浆细胞的抗人CD79a和抗人CD79b。在一例病例中，大多数肿瘤细胞同时被抗CD3和抗CD79b标记，少数被抗CD79a标记。另一例病例根据大多数肿瘤细胞被抗CD79b和抗CD79a标记而被分类为B细胞肿瘤。这是首次记录的对羊驼淋巴瘤进行免疫表型分析的尝试，据我们所知，也是兽医文献中首次记录的淋巴瘤中T细胞和B细胞相关分子假定共表达的情况。
