Krugman S
Am J Med Sci. 1975 Sep-Oct;270(2):391-3. doi: 10.1097/00000441-197509000-00023.
Studies with heat-activated serum containing hepatitis B virus (MS2 strain) revealed 1) it was not infectious, 2) it was antigenic and 3) it was protectie. Acitve immunization with this preparation was associated with the detection of antibody to the hepatitis B surface antigen (anti-HBs) and no detectable core antibody (anti-HBc). In addition, various investigators have prepared a synthetic hepatitis B peptide vaccine, These findings indicate that it should be feasible to develop an inactivated hepatitis B vaccine by the use of purified hepatitis B surface antigen or one of its polypeptides.
对含有乙肝病毒（MS2株）的热激活血清进行的研究表明：1）它没有传染性；2）它具有抗原性；3）它具有保护性。用该制剂进行主动免疫与检测到乙肝表面抗原抗体（抗-HBs）以及未检测到核心抗体（抗-HBc）相关。此外，多位研究者制备了一种合成乙肝肽疫苗。这些发现表明，利用纯化的乙肝表面抗原或其一种多肽开发一种灭活乙肝疫苗应该是可行的。
