Krugman S, Overby L R, Mushahwar I K, Ling C M, Frösner G G, Deinhardt F
N Engl J Med. 1979 Jan 18;300(3):101-6. doi: 10.1056/NEJM197901183000301.
Frozen serial serum specimens obtained from past studies on the natural history and prevention of Type B hepatitis in children were retested by radioimmunoassay for the following markers of hepatitis B infection: hepatitis B surface antigen (HBsAg) and antibody (anti-HBs), hepatitis B e antigen (HBeAg) and antibody (anti-HBe), and antibody to hepatitis B core antigen (anti-HBc). The interval between exposure and evidence of viremia (HBsAg) was as short as six days. HBsAg and HBeAg persisted for two to five months and occasionally for more than one year after recovery. After the disappearance of their respective antigens, anti-HBc and anti-HBs persisted for more than seven years and anti-HBe for one to two years. Treatment with hepatitis B immune globulin after exposure induced complete or partial protection or prolongation of the incubation period. Administration of heat-inactivated hepatitis B virus, MS-2 strain, to 29 children induced an inapparent infection in three, characterized by a transient appearance of HBsAg and HBeAg, and the persistence of anti-HBc, anti-HBe and anti-HBs for more than two years.
从过去关于儿童乙型肝炎自然史和预防的研究中获得的冷冻系列血清标本，通过放射免疫测定法重新检测乙型肝炎感染的以下标志物：乙型肝炎表面抗原（HBsAg）和抗体（抗-HBs）、乙型肝炎e抗原（HBeAg）和抗体（抗-HBe）以及乙型肝炎核心抗原抗体（抗-HBc）。暴露与病毒血症证据（HBsAg）之间的间隔短至6天。HBsAg和HBeAg在恢复后持续2至5个月，偶尔持续超过1年。各自抗原消失后，抗-HBc和抗-HBs持续超过7年，抗-HBe持续1至2年。暴露后用乙型肝炎免疫球蛋白治疗可诱导完全或部分保护或延长潜伏期。给29名儿童接种热灭活的乙型肝炎病毒MS-2株，3人发生隐性感染，其特征为HBsAg和HBeAg短暂出现，抗-HBc、抗-HBe和抗-HBs持续超过2年。
