Birtles Richard J, Fry Norman K, Ventosilla Palmira, Cáceres Abraham G, Sánchez Eduardo, Vizcarra Hugo, Raoult Didier
Department of Veterinary Pathology, Faculty of Veterinary Sciences, University of Liverpool, Neston, Cheshire CH64 7TE, UK.
J Clin Microbiol. 2002 Oct;40(10):3606-12. doi: 10.1128/JCM.40.10.3606-3612.2002.
Genotypic diversity among 26 isolates of Bartonella bacilliformis obtained from different areas of Peru, and at different times, was assessed by comparison of DNA sequences derived from 16S-23S ribosomal DNA intergenic spacer regions (ISR) and a citrate synthase gene (gltA) fragment and by amplified fragment length polymorphism (AFLP) analysis. gltA comparison divided the isolates into two groups, whereas ISR comparison revealed six sequences. AFLP analysis using a selective primer delineated five profiles that correlated well with those obtained by sequence comparison. Combination of all three data sets divided the isolates into six genotypes. One of these genotypes was common to isolates collected from a large area in western Peru that corresponded to the region of endemicity for bartonellosis; however, isolates belonging to two other genotypes were also found within this region. Two of these genotypes were found in isolates isolated more than 35 years apart. The remaining three genotypes were each specifically associated with three outbreaks of bartonellosis that have recently occurred in areas where the disease had not previously been recognized. Demonstration of the unique nature of these isolates indicates that the outbreaks with which they were associated did not result from the introduction of disease by individuals who acquired their infection in the recognized region of endemicity. The sources of these outbreaks remain unknown. A consensus approach to bacterial typing using comparative sequence analysis of multiple genetic loci and the pan-genomic sampling of AFLP appears to offer a well-supported assessment of B. bacilliformis diversity, and the genotypic differences identified appear to have epidemiological significance.
通过比较源自16S - 23S核糖体DNA基因间隔区（ISR）和柠檬酸合酶基因（gltA）片段的DNA序列以及扩增片段长度多态性（AFLP）分析，评估了从秘鲁不同地区、不同时间获得的26株杆菌状巴尔通体分离株的基因型多样性。gltA比较将分离株分为两组，而ISR比较揭示了六个序列。使用选择性引物的AFLP分析确定了五个图谱，这些图谱与通过序列比较获得的图谱相关性良好。所有三个数据集的组合将分离株分为六个基因型。其中一个基因型在从秘鲁西部大片地区收集的分离株中很常见，该地区对应于巴尔通体病的流行区域；然而，在该区域内也发现了属于其他两个基因型的分离株。在相隔35年以上分离的分离株中发现了其中两个基因型。其余三个基因型分别与最近在以前未认识到该疾病的地区发生的三次巴尔通体病暴发特别相关。这些分离株独特性质的证明表明，与它们相关的暴发并非由在公认的流行区域感染的个体引入疾病所致。这些暴发的源头仍然未知。使用多个基因座的比较序列分析和AFLP的泛基因组采样的细菌分型共识方法似乎为杆菌状巴尔通体的多样性提供了有力支持的评估，并且所确定的基因型差异似乎具有流行病学意义。
