Yamada Akiyo, Sekiguchi Mikiko, Mimura Tetsuro, Ozeki Yoshihiro
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei, Japan.
Plant Cell Physiol. 2002 Sep;43(9):1043-8. doi: 10.1093/pcp/pcf120.
To find key genes essential for salt tolerance in the mangrove plant, Bruguiera sexangula, functional screening was performed using Escherichia coli as the host organism. A transformant expressing a cytosolic chaperonin-containing TCP-1alpha (CCTalpha) homologue displayed enhanced salt tolerance. Analysis in E. coli of the functional region revealed that a sequence of only 218 amino acids, containing the apical domain, is necessary for osmotolerance. Furthermore, this domain shows chaperone activity in vitro. Therefore, CCTalpha facilitates the folding of proteins without ATP or the cage-like structure, and may play an important role in stress tolerance, at least in B. sexangula.
为了找到红树植物木榄耐盐性所必需的关键基因，以大肠杆菌作为宿主生物体进行了功能筛选。表达含细胞质伴侣蛋白TCP-1α（CCTα）同源物的转化体表现出增强的耐盐性。在大肠杆菌中对功能区域的分析表明，仅218个氨基酸的序列（包含顶端结构域）对于渗透耐受性是必需的。此外，该结构域在体外显示出伴侣活性。因此，CCTα在没有ATP或笼状结构的情况下促进蛋白质折叠，并且可能至少在木榄的胁迫耐受性中起重要作用。
