Roby D., Broglie K., Cressman R., Biddle P., Chet I. L., Broglie R.
E.I. Du Pont de Nemours & Company, Agricultural Products Department, Experimental Station, P.O. Box 80402, Wilmington, Delaware 19880.
Plant Cell. 1990 Oct;2(10):999-1007. doi: 10.1105/tpc.2.10.999.
The temporal and spatial expression of a bean chitinase promoter has been investigated in response to fungal attack. Analysis of transgenic tobacco plants containing a chimeric gene composed of a 1.7-kilobase fragment carrying the chitinase 5B gene promoter fused to the coding region of the gus A gene indicated that the chitinase promoter is activated during attack by the fungal pathogens Botrytis cinerea, Rhizoctonia solani, and Sclerotium rolfsii. Although induction of [beta]-glucuronidase activity was observed in tissues that had not been exposed to these phytopathogens, the greatest induction occurred in and around the site of fungal infection. The increase in [beta]-glucuronidase activity closely paralleled the increase in endogenous tobacco chitinase activity produced in response to fungal infection. Thus, the chitinase 5B-gus A fusion gene may be used to analyze the cellular and molecular details of the activation of the host defense system during pathogen attack.
已针对真菌侵袭对菜豆几丁质酶启动子的时空表达进行了研究。对含有嵌合基因的转基因烟草植株进行分析，该嵌合基因由携带几丁质酶5B基因启动子的1.7千碱基片段与gus A基因的编码区融合而成，结果表明几丁质酶启动子在受到真菌病原体灰葡萄孢、立枯丝核菌和齐整小核菌侵袭时被激活。虽然在未接触这些植物病原体的组织中也观察到了β-葡萄糖醛酸酶活性的诱导，但最大诱导发生在真菌感染部位及其周围。β-葡萄糖醛酸酶活性的增加与真菌感染诱导产生的内源性烟草几丁质酶活性的增加密切平行。因此，几丁质酶5B-gus A融合基因可用于分析病原体侵袭期间宿主防御系统激活的细胞和分子细节。
