Boa Zhang, Ma Wen-Li, Hu Zi-You, Rong Shi, Shi Yan-Bin, Zheng Wen-Ling
Department of Biochemistry, First Military Medical University, Guangzhou 510515, PR China.
J Biochem Mol Biol. 2002 Sep 30;35(5):532-5. doi: 10.5483/bmbrep.2002.35.5.532.
To establish a method to evaluate the quality of the printed microarray and DNA fragments' immobilization. The target gene fragments that were made with the restriction display PCR (RD-PCR) technique were printed on a superamine modified glass slide, then immobilized with UV cross-linking and heat. This chip was hybridized with universal primers that were labeled with cy3-dUTP, as well as cDNA that was labeled with cy3-dCTP, as the conventional protocol. Most of the target gene fragments on the chip showed positive signals, but the negative control showed no signal, and vice versa. We established a method that enables an effective evaluation of the quality of the microarrays.
建立一种评估打印微阵列和DNA片段固定质量的方法。用限制性显示PCR（RD-PCR）技术制备的靶基因片段被打印在经超胺修饰的载玻片上，然后通过紫外线交联和加热进行固定。按照常规方案，该芯片与用cy3-dUTP标记的通用引物以及用cy3-dCTP标记的cDNA进行杂交。芯片上的大多数靶基因片段显示出阳性信号，但阴性对照无信号，反之亦然。我们建立了一种能够有效评估微阵列质量的方法。
