Li Ling, Ma Wen Li, Zhu Ji, Shi Rong, Liu Cui Hua, Chen Jin Kun, Zheng Wen Ling
Institute of Molecular Biology, The First Uniform Medical University, Guangzhou 510515, China.
J Virol Methods. 2003 Dec;114(1):71-5. doi: 10.1016/j.jviromet.2003.09.013.
The restriction display PCR is a useful technique for studying the diversity of gene expression. This method involves ligating the digested genes with adapters and amplifying the gene fragments by PCR using universal and selective primers. In this study, we improved this restriction display PCR method by using Cy3-UP, a fluorescently labelled universal primer, in place of Cy3-dCTP in sample-labelling for DNA microarray. The results show that this new method increases significantly the sensitivity of the assay, and will have a wide application in the DNA microarray field.
限制性显示PCR是一种用于研究基因表达多样性的有用技术。该方法包括将消化后的基因与接头连接，并使用通用引物和选择性引物通过PCR扩增基因片段。在本研究中，我们通过使用Cy3-UP（一种荧光标记的通用引物）代替DNA微阵列样品标记中的Cy3-dCTP，改进了这种限制性显示PCR方法。结果表明，这种新方法显著提高了检测的灵敏度，并将在DNA微阵列领域得到广泛应用。
