Lin Ying, Huang Ruochun, Santanam Nalini, Liu Ya-Guang, Parthasarathy Sampath, Huang Ruo-Pan
Department of Gynecology and Obstetrics, Emory University School of Medicine, 1639 Pierce Drive, Room 429, Atlanta, GA 30322, USA.
Cancer Lett. 2002 Dec 10;187(1-2):17-24. doi: 10.1016/s0304-3835(02)00346-4.
Previously, we demonstrated that vitamin E supplementation decreases autoantibodies to oxidized lipid-protein complexes (J. Med. Food 1 (2000) 247). Utilizing an in vitro modeling system, we also demonstrated that vitamin E blocks the tumor promotion process in liver epithelial cells (Carcinogenesis 20 (1999) 485 and Mol. Carcinog. 30 (2001) 209). To investigate the molecular mechanisms of vitamin E function, we developed a human cytokine array system that is capable of detecting the expression of 35 cytokines simultaneously. Using this new technology, we analyzed the potential vitamin E-regulated cytokines in vitamin E supplementation individuals. The cytokine arrays showed that expression of several cytokines, particularly monocyte chemoattractant protein-1 (MCP-1), was profoundly reduced in vitamin E supplementation individuals. Moreover, addition of vitamin E to several cultured cells significantly down-regulated the expression of MCP-1. Our results suggested that MCP-1 may be one of the most important targets of antioxidant vitamin E. To the best of our knowledge, this is the first report describing the down-regulation of MCP-1 in vitamin E supplementation in vivo.
此前，我们证明补充维生素E可降低针对氧化脂质 - 蛋白质复合物的自身抗体（《医学食品杂志》1（2000年）247）。利用体外建模系统，我们还证明维生素E可阻断肝上皮细胞中的肿瘤促进过程（《癌变》20（1999年）485以及《分子致癌作用》30（2001年）209）。为研究维生素E功能的分子机制，我们开发了一种能够同时检测35种细胞因子表达的人细胞因子阵列系统。利用这项新技术，我们分析了补充维生素E个体中潜在的受维生素E调节的细胞因子。细胞因子阵列显示，在补充维生素E的个体中，几种细胞因子的表达，尤其是单核细胞趋化蛋白 -1（MCP -1），显著降低。此外，向几种培养细胞中添加维生素E可显著下调MCP -1的表达。我们的结果表明，MCP -1可能是抗氧化维生素E最重要的靶点之一。据我们所知，这是第一份描述体内补充维生素E时MCP -1下调的报告。
