Ciba-Geigy Biotechnology, P. O. Box 12257, Research Triangle Park, NC 27709.
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8439-43. doi: 10.1073/pnas.84.23.8439.
Antisense nopaline synthase (nos) (D-nopaline synthase; EC 1.5.1.19) RNA is stably expressed from the cauliflower mosaic virus 35S promoter in transformed tobacco plants. The expression of a previously introduced wild-type nos gene is inhibited by the antisense RNA, with less nos enzyme activity detected (by a factor of 8-50) depending on the tissue analyzed. The steady-state levels of nos mRNA are reduced in the presence of the antisense RNA, implying that mRNA degradation is probably the main mode of action for the decrease in expression in this system. The antisense RNA-expressing gene and its inhibition of nos expression are shown to be heritable, demonstrating that it is a potentially useful method for the modification of phenotype.
反义胭脂碱合成酶（nos）（D-胭脂碱合成酶；EC 1.5.1.19）RNA 可在转化的烟草植物中稳定地由花椰菜花叶病毒 35S 启动子表达。引入的野生型 nos 基因的表达被反义 RNA 抑制，根据分析的组织不同，检测到的 nos 酶活性降低（降低 8-50 倍）。在反义 RNA 的存在下，nos mRNA 的稳态水平降低，这意味着在该系统中表达降低的主要作用方式可能是 mRNA 降解。反义 RNA 表达基因及其对 nos 表达的抑制作用是可遗传的，表明它是一种用于修饰表型的潜在有用方法。
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