Theerakulpisut P, Xu H, Singh M B, Pettitt J M, Knox R B
School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Plant Cell. 1991 Oct;3(10):1073-84. doi: 10.1105/tpc.3.10.1073.
Differential screening of a mature Brassica campestris pollen cDNA library has identified five cDNA clones that represent transcripts expressed exclusively, or at elevated levels, in pollen. We show here that the expression of one of these, clone Bcp1, is tissue specific and temporally regulated. The gene is activated during microspore development, as detected by in situ hybridization. Expression is enhanced at the time of pollen maturation and during pollen germination. In situ hybridization has also shown that Bcp 1 is activated in the tapetal cells in early anther development and continues to be expressed until tapetal dissolution. Homologous transcripts are present in pollen of other taxa of Brassicaceae including Arabidopsis, but not in pollen of any other families tested.
对一个成熟的白菜花粉cDNA文库进行差异筛选，鉴定出了五个cDNA克隆，它们代表了仅在花粉中表达或在花粉中高水平表达的转录本。我们在此表明，其中一个克隆Bcp1的表达具有组织特异性且受时间调控。通过原位杂交检测发现，该基因在小孢子发育过程中被激活。在花粉成熟时和花粉萌发期间表达增强。原位杂交还表明，Bcp1在花药发育早期的绒毡层细胞中被激活，并持续表达直至绒毡层解体。在包括拟南芥在内的十字花科其他类群的花粉中存在同源转录本，但在所测试的任何其他科的花粉中均不存在。
