Hu Dan-Ning, Ritch Robert, Liebmann Jeffrey, Liu Yizhi, Cheng Bin, Hu Mark S
Tissue Culture Center, Department of Pathology and Laboratory Medicine, The New York Eye and Ear Infirmary, New York, New York 10003, USA.
J Glaucoma. 2002 Oct;11(5):406-10. doi: 10.1097/00061198-200210000-00006.
To assess the concentrations of vascular endothelial growth factor (VEGF) in aqueous humor in eyes with and without glaucoma.
Concentrations of VEGF were measured using a sandwich ELISA kit in aqueous humor aspirates taken during anterior segment surgery from 87 patients, of whom 54 had glaucoma (27 primary open-angle glaucoma, 8 angle-closure glaucoma, 16 exfoliative glaucoma) and 33 had cataract only.
Vascular endothelial growth factor was detected in all samples. The concentration in eyes with cataract only without glaucoma was 102.4 +/- 29.7 pg/mL (mean +/- SD), which was significantly lower than that from eyes with glaucoma (146.7 +/- 51.8 pg/mL). There were no significant differences between primary open-angle glaucoma (140.4 +/- 51.0 pg/mL), angle-closure glaucoma (142.8 +/- 40.2 pg/mL), and exfoliative glaucoma (158.6 +/- 58.9 pg/mL). An unusually high VEGF concentration was detected in one eye with neovascular glaucoma (759 pg/mL) and two eyes with uveitic glaucoma (322 pg/mL). No effect of age, gender, or previous history of medical, laser, or surgical treatment of the aqueous humor VEGF concentration could be detected ( > 0.05). Aqueous humor and plasma VEGF concentrations were measured and compared in 46 patients. The aqueous humor VEGF concentration (144.2 +/- 107.9 pg/mL) was significantly higher ( < 0.01) than the plasma concentration (79.2 +/- 46.1 pg/mL). No significant correlation was found between aqueous humor and plasma VEGF concentrations.
Aqueous VEGF concentration is increased in eyes with glaucoma.
评估有青光眼和无青光眼患者房水中血管内皮生长因子（VEGF）的浓度。
采用夹心酶联免疫吸附测定试剂盒，对87例患者眼前节手术时采集的房水抽吸物中的VEGF浓度进行测量，其中54例患有青光眼（27例原发性开角型青光眼、8例闭角型青光眼、16例剥脱性青光眼），33例仅患有白内障。
所有样本均检测到血管内皮生长因子。仅患有白内障而无青光眼的患者眼中VEGF浓度为102.4±29.7 pg/mL（平均值±标准差），显著低于青光眼患者眼中的浓度（146.7±51.8 pg/mL）。原发性开角型青光眼（140.4±51.0 pg/mL）、闭角型青光眼（142.8±40.2 pg/mL）和剥脱性青光眼（158.6±58.9 pg/mL）之间无显著差异。在1例新生血管性青光眼患者眼中检测到异常高的VEGF浓度（759 pg/mL），在2例葡萄膜炎性青光眼患者眼中检测到异常高的VEGF浓度（322 pg/mL）。未检测到年龄、性别或房水VEGF浓度的既往药物、激光或手术治疗史对其有影响（P>0.05）。对46例患者的房水和血浆VEGF浓度进行了测量和比较。房水VEGF浓度（144.2±107.9 pg/mL）显著高于血浆浓度（79.2±46.1 pg/mL）（P<0.01）。房水和血浆VEGF浓度之间未发现显著相关性。
青光眼患者房水中VEGF浓度升高。
