青光眼患者房水中血管内皮生长因子水平升高。

Vascular endothelial growth factor is increased in aqueous humor of glaucomatous eyes.

作者信息

Hu Dan-Ning, Ritch Robert, Liebmann Jeffrey, Liu Yizhi, Cheng Bin, Hu Mark S

机构信息

Tissue Culture Center, Department of Pathology and Laboratory Medicine, The New York Eye and Ear Infirmary, New York, New York 10003, USA.

出版信息

J Glaucoma. 2002 Oct;11(5):406-10. doi: 10.1097/00061198-200210000-00006.

DOI:10.1097/00061198-200210000-00006

PMID:12362079

Abstract

PURPOSE

To assess the concentrations of vascular endothelial growth factor (VEGF) in aqueous humor in eyes with and without glaucoma.

METHODS

Concentrations of VEGF were measured using a sandwich ELISA kit in aqueous humor aspirates taken during anterior segment surgery from 87 patients, of whom 54 had glaucoma (27 primary open-angle glaucoma, 8 angle-closure glaucoma, 16 exfoliative glaucoma) and 33 had cataract only.

RESULTS

Vascular endothelial growth factor was detected in all samples. The concentration in eyes with cataract only without glaucoma was 102.4 +/- 29.7 pg/mL (mean +/- SD), which was significantly lower than that from eyes with glaucoma (146.7 +/- 51.8 pg/mL). There were no significant differences between primary open-angle glaucoma (140.4 +/- 51.0 pg/mL), angle-closure glaucoma (142.8 +/- 40.2 pg/mL), and exfoliative glaucoma (158.6 +/- 58.9 pg/mL). An unusually high VEGF concentration was detected in one eye with neovascular glaucoma (759 pg/mL) and two eyes with uveitic glaucoma (322 pg/mL). No effect of age, gender, or previous history of medical, laser, or surgical treatment of the aqueous humor VEGF concentration could be detected ( > 0.05). Aqueous humor and plasma VEGF concentrations were measured and compared in 46 patients. The aqueous humor VEGF concentration (144.2 +/- 107.9 pg/mL) was significantly higher ( < 0.01) than the plasma concentration (79.2 +/- 46.1 pg/mL). No significant correlation was found between aqueous humor and plasma VEGF concentrations.

CONCLUSION

Aqueous VEGF concentration is increased in eyes with glaucoma.

摘要

目的

评估有青光眼和无青光眼患者房水中血管内皮生长因子（VEGF）的浓度。

方法

采用夹心酶联免疫吸附测定试剂盒，对87例患者眼前节手术时采集的房水抽吸物中的VEGF浓度进行测量，其中54例患有青光眼（27例原发性开角型青光眼、8例闭角型青光眼、16例剥脱性青光眼），33例仅患有白内障。

结果

所有样本均检测到血管内皮生长因子。仅患有白内障而无青光眼的患者眼中VEGF浓度为102.4±29.7 pg/mL（平均值±标准差），显著低于青光眼患者眼中的浓度（146.7±51.8 pg/mL）。原发性开角型青光眼（140.4±51.0 pg/mL）、闭角型青光眼（142.8±40.2 pg/mL）和剥脱性青光眼（158.6±58.9 pg/mL）之间无显著差异。在1例新生血管性青光眼患者眼中检测到异常高的VEGF浓度（759 pg/mL），在2例葡萄膜炎性青光眼患者眼中检测到异常高的VEGF浓度（322 pg/mL）。未检测到年龄、性别或房水VEGF浓度的既往药物、激光或手术治疗史对其有影响（P>0.05）。对46例患者的房水和血浆VEGF浓度进行了测量和比较。房水VEGF浓度（144.2±107.9 pg/mL）显著高于血浆浓度（79.2±46.1 pg/mL）（P<0.01）。房水和血浆VEGF浓度之间未发现显著相关性。