Tripathi R C, Li J, Tripathi B J, Chalam K V, Adamis A P
Department of Ophthalmology, University of South Carolina School of Medicine, Columbia, USA.
Ophthalmology. 1998 Feb;105(2):232-7. doi: 10.1016/s0161-6420(98)92782-8.
This study aimed to quantitate and compare the concentration of vascular endothelial growth factor (VEGF) in aqueous humor samples from patients with neovascular glaucoma (NVG), primary open-angle glaucoma (POAG), and cataract, as well as in serum samples of healthy human subjects.
The authors collected aqueous humor samples by using their previously published technique of limbal paracentesis. The authors determined the concentration of VEGF by using a competitive enzyme immunoassay system and four-parameter logistic curve fitting and performed statistical analysis by using the Mann-Whitney-Wilcoxon test.
The authors detected VEGF in 12 of 12 samples from patients with NVG (mean +/- standard error of the mean, 29.267 +/- 7.350 ng/ml), 15 of 28 samples from patients with POAG (0.726 +/- 0.204 ng/ml), 4 of 20 aqueous humor samples from patients with cataract (0.257 +/- 0.043 ng/ml), and 16 of 16 human serum samples (20.246 +/- 1.568 ng/ml). The mean concentration of VEGF in aqueous humor of patients with NVG was 40- and 113-fold higher than that in patients with POAG and cataract, respectively, and the difference was statistically significant (P < 0.01). The VEGF level in patients with POAG was elevated compared with that in patients with cataract (P < 0.05). Although the mean concentration of VEGF in aqueous humor of patients with NVG was approximately 1.45-fold higher than that in serum, the difference was not significant (P > 0.05).
The authors' findings show that patients with NVG had a significantly increased level of VEGF in the aqueous humor and implicate VEGF as an important factor in the pathogenesis of intraocular neovascularization in these patients. The authors discuss the possible role of the ciliary epithelium, in addition to retina, in the production of VEGF and the complementary function of basic fibroblast growth factor and other growth factors.
本研究旨在定量并比较新生血管性青光眼（NVG）、原发性开角型青光眼（POAG）和白内障患者房水样本以及健康人血清样本中血管内皮生长因子（VEGF）的浓度。
作者采用其先前发表的角膜缘穿刺技术收集房水样本。作者使用竞争性酶免疫分析系统和四参数逻辑曲线拟合测定VEGF浓度，并采用Mann-Whitney-Wilcoxon检验进行统计分析。
作者在12例NVG患者的12份样本中检测到VEGF（平均±均值标准误，29.267±7.350 ng/ml），28例POAG患者的15份样本中（0.726±0.204 ng/ml），20例白内障患者的4份房水样本中（0.257±0.043 ng/ml），以及16例人血清样本的16份中（20.246±1.568 ng/ml）检测到VEGF。NVG患者房水中VEGF的平均浓度分别比POAG和白内障患者高40倍和113倍，差异具有统计学意义（P<0.01）。POAG患者的VEGF水平高于白内障患者（P<0.05）。尽管NVG患者房水中VEGF的平均浓度比血清中高约1.45倍，但差异不显著（P>0.05）。
作者的研究结果表明，NVG患者房水中VEGF水平显著升高，提示VEGF是这些患者眼内新生血管形成发病机制中的一个重要因素。作者讨论了睫状体上皮除视网膜外，在VEGF产生中的可能作用以及碱性成纤维细胞生长因子和其他生长因子的互补功能。
