Kissane J Q, Hawrylewicz E J
Pediatr Res. 1975 Mar;9(3):146-50. doi: 10.1203/00006450-197503000-00008.
Four protein dietary groups of rats were used in this study, namely: 27% casein diet fed throughout gestation and lactation (group C-C); 27% gestation, 10% lactation (group C-D); 10% gestation, 27% lactation (group D-C); 10% throughout gestation and lactation (group D-D). Mean body weight of group D-D at warning was 19 g compared with 59 g for group C-C. The specific activity of Na+-K+-ATPase in isolated synaptosomes from cerebral and cerebellar tissues increases throughout the lactation period. At 1 and 7 days of age no differences in Na+-K+-ATPase activity are noted in the brain regions of Groups C-C and D-D. At 14 and 21 days, however, the specific Na+-K+-ATPase activity was significantly reduced in isolated synaptosomes from both brain regions of the protein-deprived group. ATPase activity in cerebellar synaptosomes from dietary exchange groups (C-D and D-C) developed to the same extent as that of the control group (C-C) after 21 days of lactation. ATPase activity in the cerebral synaptosomes of the dietary exchange group (C-D) did not develop to the same extent as that in the control group (C-C) and the other dietary exchange group (D-C).
本研究使用了四组蛋白质饮食的大鼠，即：在整个妊娠期和哺乳期喂食27%酪蛋白饮食的大鼠（C-C组）；妊娠期27%、哺乳期10%的大鼠（C-D组）；妊娠期10%、哺乳期27%的大鼠（D-C组）；在整个妊娠期和哺乳期均为10%的大鼠（D-D组）。断奶时D-D组大鼠的平均体重为19克，而C-C组为59克。在整个哺乳期，从大脑和小脑组织分离出的突触体中Na+-K+-ATP酶的比活性都会增加。在1日龄和7日龄时，C-C组和D-D组大鼠的脑区中Na+-K+-ATP酶活性没有差异。然而，在14日龄和21日龄时，蛋白质缺乏组两个脑区分离出的突触体中Na+-K+-ATP酶的比活性显著降低。哺乳期21天后，饮食交换组（C-D组和D-C组）小脑突触体中的ATP酶活性发展到与对照组（C-C组）相同的程度。饮食交换组（C-D组）大脑突触体中的ATP酶活性没有发展到与对照组（C-C组）和另一个饮食交换组（D-C组）相同的程度。
